Can Be miR-126-3p a Biomarker of Premature Aging? An Ex Vivo and In Vitro Study in Fabry Disease

Calogero Caruso, Tiziana Di Chiara, Giuseppina Candore, Riccardo Alessandro, Giulia Accardi, Anna Aiello, Tiziana Di Chiara, Simona Taverna, Maria Assunta Costa, Giuseppa Augello, Daniele Francofonte, Carmela Zizzo, Giorgia Adamo, Alessia Lo Curto, Alessia Lo Curto, Marco Zora, Maria Assunta Costa, Maria Assunta Costa, Paolo Colomba, Rosa PassantinoTiziana Di Chiara, Giovanni Duro, Riccardo Alessandro, Giuseppe Cammarata, Giuseppe Cammarata

Risultato della ricerca: Articlepeer review

1 Citazioni (Scopus)

Abstract

Fabry disease (FD) is a lysosomal storage disorder (LSD) characterized by lysosomal accumulation of glycosphingolipids in a wide variety of cytotypes, including endothelial cells (ECs). FD patients experience a significantly reduced life expectancy compared to the general population; therefore, the association with a premature aging process would be plausible. To assess this hypothesis, miR-126-3p, a senescence-associated microRNA (SA-miRNAs), was considered as an aging biomarker. The levels of miR-126-3p contained in small extracellular vesicles (sEVs), with about 130 nm of diameter, were measured in FD patients and healthy subjects divided into age classes, in vitro, in human umbilical vein endothelial cells (HUVECs) "young" and undergoing replicative senescence, through a quantitative polymerase chain reaction (qPCR) approach. We confirmed that, in vivo, circulating miR-126 levels physiologically increase with age. In vitro, miR-126 augments in HUVECs underwent replicative senescence. We observed that FD patients are characterized by higher miR-126-3p levels in sEVs, compared to age-matched healthy subjects. We also explored, in vitro, the effect on ECs of glycosphingolipids that are typically accumulated in FD patients. We observed that FD storage substances induced in HUVECs premature senescence and increased of miR-126-3p levels. This study reinforces the hypothesis that FD may aggravate the normal aging process.
Lingua originaleEnglish
pagine (da-a)1-16
Numero di pagine16
RivistaCells
Volume10
Stato di pubblicazionePublished - 2021

All Science Journal Classification (ASJC) codes

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