Cadmium induces the expression of specific stress proteins in sea urchin embryos

Maria Carmela Roccheri, Rosa Bonaventura, Valeria Matranga, Maria Agnello

Risultato della ricerca: Article

67 Citazioni (Scopus)

Abstract

Marine organisms are highly sensitive to many environmental stresses, and consequently, the analysis of their bio-molecular responses to different stress agents is very important for the understanding of putative repair mechanisms. Sea urchin embryos represent a simple though significant model system to test how specific stress can simultaneously affect development and protein expression. Here, we used Paracentrotus lividus sea urchin embryos to study the effects of time-dependent continuous exposure to subacute/sublethal cadmium concentrations. We found that, between 15 and 24 h of exposure, the synthesis of a specific set of stress proteins (90, 72-70, 56, 28, and 25 kDa) was induced, with an increase in the rate of synthesis of 72-70 kDa (hsps), 56 kDa (hsp), and 25 kDa, which was dependent on the lengths of treatment. Recovery experiments in which cadmium was removed showed that while stress proteins continued to be synthesized, embryo development was resumed only after short lengths of exposure.
Lingua originaleEnglish
pagine (da-a)80-87
Numero di pagine8
RivistaBiochemical and Biophysical Research Communications
Volume321
Stato di pubblicazionePublished - 2004

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Sea Urchins
Heat-Shock Proteins
Cadmium
Embryonic Structures
Paracentrotus
Aquatic Organisms
Exercise Test
Embryonic Development
Repair
Recovery
Proteins
Experiments

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Cadmium induces the expression of specific stress proteins in sea urchin embryos. / Roccheri, Maria Carmela; Bonaventura, Rosa; Matranga, Valeria; Agnello, Maria.

In: Biochemical and Biophysical Research Communications, Vol. 321, 2004, pag. 80-87.

Risultato della ricerca: Article

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AB - Marine organisms are highly sensitive to many environmental stresses, and consequently, the analysis of their bio-molecular responses to different stress agents is very important for the understanding of putative repair mechanisms. Sea urchin embryos represent a simple though significant model system to test how specific stress can simultaneously affect development and protein expression. Here, we used Paracentrotus lividus sea urchin embryos to study the effects of time-dependent continuous exposure to subacute/sublethal cadmium concentrations. We found that, between 15 and 24 h of exposure, the synthesis of a specific set of stress proteins (90, 72-70, 56, 28, and 25 kDa) was induced, with an increase in the rate of synthesis of 72-70 kDa (hsps), 56 kDa (hsp), and 25 kDa, which was dependent on the lengths of treatment. Recovery experiments in which cadmium was removed showed that while stress proteins continued to be synthesized, embryo development was resumed only after short lengths of exposure.

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