TY - JOUR
T1 - C1q as a unique player in angiogenesis with therapeutic implication in wound healing
AU - Tripodo, Claudio
AU - Guarnotta, Carla
AU - Agostinis, Chiara
AU - Rizzi, Lucia
AU - Ling, Guang Sheng
AU - Tedesco, Francesco
AU - Ling, Guang Sheng
AU - Zorzet, Sonia
AU - Munaut, Carine
AU - Botto, Marina
AU - Baldassarre, Gustavo
AU - Ghebrehiwet, Berhane
AU - Bossi, Fleur
AU - Bulla, Roberta
AU - Papa, Giovanni
PY - 2014
Y1 - 2014
N2 - We have previously shown that C1q is expressed on endothelial cells (ECs) of newly formed decidual tissue. Here we demonstrate that C1q is deposited in wound-healing skin in the absence of C4 and C3 and that C1q mRNA is locally expressed as revealed by real-time PCR and in situ hybridization. C1q was found to induce permeability of the EC monolayer, to stimulate EC proliferation and migration, and to promote tube formation and sprouting of new vessels in a rat aortic ring assay. Using a murine model of wound healing we observed that vessel formation was defective in C1qa(-/-) mice and was restored to normal after local application of C1q. The mean vessel density of wound-healing tissue and the healed wound area were significantly increased in C1q-treated rats. On the basis of these results we suggest that C1q may represent a valuable therapeutic agent that can be used to treat chronic ulcers or other pathological conditions in which angiogenesis is impaired, such as myocardial ischemia.
AB - We have previously shown that C1q is expressed on endothelial cells (ECs) of newly formed decidual tissue. Here we demonstrate that C1q is deposited in wound-healing skin in the absence of C4 and C3 and that C1q mRNA is locally expressed as revealed by real-time PCR and in situ hybridization. C1q was found to induce permeability of the EC monolayer, to stimulate EC proliferation and migration, and to promote tube formation and sprouting of new vessels in a rat aortic ring assay. Using a murine model of wound healing we observed that vessel formation was defective in C1qa(-/-) mice and was restored to normal after local application of C1q. The mean vessel density of wound-healing tissue and the healed wound area were significantly increased in C1q-treated rats. On the basis of these results we suggest that C1q may represent a valuable therapeutic agent that can be used to treat chronic ulcers or other pathological conditions in which angiogenesis is impaired, such as myocardial ischemia.
UR - http://hdl.handle.net/10447/96425
M3 - Article
SN - 0027-8424
VL - 111
SP - 4209
EP - 4214
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
ER -