Buffy coat-derived platelets cryopreserved using a new method: Results from in vitro studies

Francesco Dieli, Sergio Siragusa, Nadia Rosalia Caccamo, Salvatrice Mancuso, Mariasanta Napolitano, Simona Raso, Rosalia Agliastro, Giovanni De Francisci, Amalia Reina, Lucio Lo Coco, Alberto Dolce, Giovanni De Francisci, Alberto Dolce, Lucio Lo Coco, Piera Stefania Arfo', Rosalia Agliastro

Risultato della ricerca: Articlepeer review

4 Citazioni (Scopus)

Abstract

Cryopreservation for the long-term storage of platelets (PLTs) is a useful method to overcome the limits of platelet shortage. This is an in vitro prospective study to evaluate the count, viability, and function of buffy coat-derived pooled platelet concentrates (BC-PLTs), treated with dimethyl sulphoxide (DMSO) and cryopreserved (CRY BC-PLTs) at −80 °C with a modified Valeri method. PLTs were stored in 6% DMSO with a patented kit. Overall, 49 BC-PLTs from 245 healthy volunteer donors were prepared, cryopreserved, and analysed before and after 3, 6, and 9 months of storage. In flow cytometry, a statistically significant reduction in CD 42b (92.7 ± 4.29% at T0 vs. 23.6 ± 27.5% at T3, 16.38 ± 12.54% at T6, and 17.3 ± 9.6% at T9) and PAC-1 (1.9 ± 1.34% at T0 vs. 0.62 ± 0.4% at T3, 0.63 ± 0.83% at T6, and 0.49 ± 0.48% at T9) was observed after storage. CRY BC-PLTs showed a good and stable endogenous thrombin generation potential (nM min): 529.25 ± 98.64 at T0 vs. 533.04 ± 103.15 at T9 months. CRY BC-PLTs showed a good viability in vitro, according to currently accepted criteria for cryopreserved PLTs.
Lingua originaleEnglish
Numero di pagine4
RivistaTransfusion and Apheresis Science
Stato di pubblicazionePublished - 2018

All Science Journal Classification (ASJC) codes

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