Brain histamine depletion enhances motor sequences complexity of mice tested in open field: new insights from temporal pattern analysis

Risultato della ricerca: Other contribution


Background: Tourette Syndrome (TS) has been classically related to dopaminergic dysregulation of Basal Ganglia circuitry [1].However, the identification of a rare autosomal dominant form of TS, linked to a mutation of the key-enzyme for Histamine (HA)1 synthesis (l-Histidine decarboxylase, Hdc), has shed light on a putative involvement of this monoamine on the pathophysiology of tic and related disorders and fostered the development of coherent animal models [2]. Although HA-deficient rodents display markedly increased tic-like stereotypies following amphetamine administration, neither underlying nor predisposing differences in the open field test have been reported in quantitative measurements of their baseline behaviour, compared to controls [3]. Aim: To investigate, by means of multivariate Temporal Pattern Analysis, the effect of an acute HA depletion on the temporal organization of behavioural motor sequences of mice tested in the open field. Methods: We employed 10 adult HA deprived CD1 mice tested for 5 minutes in the open field and 10 controls (Ctrl). Histamine Deprivation (HD) was obtained via i.c.v. administration of 5mg alpha-fluoromethylhistidine (aFMH), a suicide inhibitor of Hdc, 24 hours before the test. Ctrl group received i.c.v. injections of saline. Experiments were recorded, and video files were stored in a PC for the following hand-made coding process. To this purpose an ethogram encompassing nine behavioural components was employed. Temporal Pattern Analysis was carried out as it represents a refined tool to determine whether two or more behavioural components occur sequentially and with statistically significant time intervals, even in the absence of evident motor stereotypies. These sequences are named temporal patterns [4].Results: An average number for subject of 229.7 (HD group) and 238.8 (Ctrl group) behavioural components were detected. Quantitative analyses with T-test for unpaired samples showed no difference between the two groups in both durations and frequencies of each component of the ethogram. However, bymeans of Temporal Pattern Analysis, the HD group revealed an evident increase in the complexity of the repeated temporal patterns compared to the Ctrl group. We found 34 patterns of different composition occurring, comprehensively, 3654 times in the HD group. Significantly, the Ctrl group showed 17 differentpatterns occurring 2157 times. In addition, HD was associated with patterns encompassing up to 11 behavioural components, whereas Ctrl with patterns limited to 4. Most of the detected patterns represent vertical and horizontal exploratory activity. Fourteen specific patterns were found in both groups. Amongthese, five represent sequences of grooming activity. Conclusion: HD seems to be related with a general enhancement of pattern complexity in number and structure. Our results suggest that HA could exert an inhibitory modulation on the expression of specific temporal patterns characterizing mice behavioralphenotype in the open field. This study is in line with recent data suggesting a putative role of HA in the pathophysiology of tics and related disorders [5]. HA dysfunction within the CNS could result in a reduced motor selectivity and thus facilitate the appearance of stereotypies under dopamine stimulation. Furtheranalyses are underway in our laboratoriesReferences[1] Leckman, J.F., Bloch, M.H., Smith, M.E., Larabi, D., Hampson, M., 2010. Neurobiological substrates of Tourette’s disorder. J Child Adolesc Psychopharmacol. 20 (4), 237-247.[2] Ercan-Sencicek, A.G., Stillman, A.A., Ghosh, A.K.,
Lingua originaleEnglish
Stato di pubblicazionePublished - 2016


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