Biotechnological Applications for the Sustainable Use of Marine By-products: In Vitro Antioxidant and Pro-apoptotic Effects of Astaxanthin Extracted with Supercritical CO 2 from Parapeneus longirostris

Concetta Maria Messina, Andrea Santulli, Andrea Santulli, Giuseppe Renda, Simona Manuguerra

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Abstract

In this study, the carotenoid astaxanthin was obtained by supercritical fluid extraction (SFE) from shrimp by-products (SBP). Itsbioactive properties were evaluated in vitro in human normal and cancerous cells lines. The antioxidant activity of the extractedastaxanthin of the SFE fraction (ASTA) was tested in fibroblast cells (HS-68), by inducing oxidative stress and by evaluating theprotective effect of the pre-treatment with different levels of ASTA against toxicity. The anti-proliferative activity was evaluatedin hepatoma cells (HEP-G2), treated with increased concentrations of ASTA and measuring the effects on vitality and on somebiomolecular markers related to oxidative stress, cell cycle, and apoptosis. It was found that pre-treating normal fibroblast cellswith ASTA resulted in a marked increase in cell viability in a dose-dependent manner (P < 0.05) attesting its antioxidant power;in cancer cell line, increased concentrations of ASTA caused a time-dose-dependent decrease in the vitality, attesting its antiproliferativeactivity (P < 0.05). The increased levels of the protein p-53 and the reduced levels of the proteins c-Jun and c-Fos athigher concentrations of ASTA, as well as, suggest the pro-apoptotic and anti-cancerous effects that this extract has on hepatocellularcarcinomas, confirmed also by caspase-3 activation. These findings suggest biotechnological utilisation of marine byproductsfor nutraceutical and pharmaceutical applications avoiding the employment of organic solvents for extraction.
Lingua originaleEnglish
pagine (da-a)565-576
Numero di pagine12
RivistaMarine Biotechnology
Volume21
Stato di pubblicazionePublished - 2019

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Supercritical Fluid Chromatography
supercritical fluid extraction
astaxanthin
Carbon Monoxide
antioxidant
fibroblasts
Job Application
Oxidative Stress
oxidative stress
Fibroblasts
Antioxidants
cell lines
Proto-Oncogene Proteins c-jun
antioxidants
Cell Line
caspase-3
hepatoma
Carotenoids
dosage
Dietary Supplements

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Aquatic Science

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title = "Biotechnological Applications for the Sustainable Use of Marine By-products: In Vitro Antioxidant and Pro-apoptotic Effects of Astaxanthin Extracted with Supercritical CO 2 from Parapeneus longirostris",
abstract = "In this study, the carotenoid astaxanthin was obtained by supercritical fluid extraction (SFE) from shrimp by-products (SBP). Itsbioactive properties were evaluated in vitro in human normal and cancerous cells lines. The antioxidant activity of the extractedastaxanthin of the SFE fraction (ASTA) was tested in fibroblast cells (HS-68), by inducing oxidative stress and by evaluating theprotective effect of the pre-treatment with different levels of ASTA against toxicity. The anti-proliferative activity was evaluatedin hepatoma cells (HEP-G2), treated with increased concentrations of ASTA and measuring the effects on vitality and on somebiomolecular markers related to oxidative stress, cell cycle, and apoptosis. It was found that pre-treating normal fibroblast cellswith ASTA resulted in a marked increase in cell viability in a dose-dependent manner (P < 0.05) attesting its antioxidant power;in cancer cell line, increased concentrations of ASTA caused a time-dose-dependent decrease in the vitality, attesting its antiproliferativeactivity (P < 0.05). The increased levels of the protein p-53 and the reduced levels of the proteins c-Jun and c-Fos athigher concentrations of ASTA, as well as, suggest the pro-apoptotic and anti-cancerous effects that this extract has on hepatocellularcarcinomas, confirmed also by caspase-3 activation. These findings suggest biotechnological utilisation of marine byproductsfor nutraceutical and pharmaceutical applications avoiding the employment of organic solvents for extraction.",
author = "Messina, {Concetta Maria} and Andrea Santulli and Andrea Santulli and Giuseppe Renda and Simona Manuguerra",
year = "2019",
language = "English",
volume = "21",
pages = "565--576",
journal = "Marine Biotechnology",
issn = "1436-2228",
publisher = "Springer New York",

}

TY - JOUR

T1 - Biotechnological Applications for the Sustainable Use of Marine By-products: In Vitro Antioxidant and Pro-apoptotic Effects of Astaxanthin Extracted with Supercritical CO 2 from Parapeneus longirostris

AU - Messina, Concetta Maria

AU - Santulli, Andrea

AU - Santulli, Andrea

AU - Renda, Giuseppe

AU - Manuguerra, Simona

PY - 2019

Y1 - 2019

N2 - In this study, the carotenoid astaxanthin was obtained by supercritical fluid extraction (SFE) from shrimp by-products (SBP). Itsbioactive properties were evaluated in vitro in human normal and cancerous cells lines. The antioxidant activity of the extractedastaxanthin of the SFE fraction (ASTA) was tested in fibroblast cells (HS-68), by inducing oxidative stress and by evaluating theprotective effect of the pre-treatment with different levels of ASTA against toxicity. The anti-proliferative activity was evaluatedin hepatoma cells (HEP-G2), treated with increased concentrations of ASTA and measuring the effects on vitality and on somebiomolecular markers related to oxidative stress, cell cycle, and apoptosis. It was found that pre-treating normal fibroblast cellswith ASTA resulted in a marked increase in cell viability in a dose-dependent manner (P < 0.05) attesting its antioxidant power;in cancer cell line, increased concentrations of ASTA caused a time-dose-dependent decrease in the vitality, attesting its antiproliferativeactivity (P < 0.05). The increased levels of the protein p-53 and the reduced levels of the proteins c-Jun and c-Fos athigher concentrations of ASTA, as well as, suggest the pro-apoptotic and anti-cancerous effects that this extract has on hepatocellularcarcinomas, confirmed also by caspase-3 activation. These findings suggest biotechnological utilisation of marine byproductsfor nutraceutical and pharmaceutical applications avoiding the employment of organic solvents for extraction.

AB - In this study, the carotenoid astaxanthin was obtained by supercritical fluid extraction (SFE) from shrimp by-products (SBP). Itsbioactive properties were evaluated in vitro in human normal and cancerous cells lines. The antioxidant activity of the extractedastaxanthin of the SFE fraction (ASTA) was tested in fibroblast cells (HS-68), by inducing oxidative stress and by evaluating theprotective effect of the pre-treatment with different levels of ASTA against toxicity. The anti-proliferative activity was evaluatedin hepatoma cells (HEP-G2), treated with increased concentrations of ASTA and measuring the effects on vitality and on somebiomolecular markers related to oxidative stress, cell cycle, and apoptosis. It was found that pre-treating normal fibroblast cellswith ASTA resulted in a marked increase in cell viability in a dose-dependent manner (P < 0.05) attesting its antioxidant power;in cancer cell line, increased concentrations of ASTA caused a time-dose-dependent decrease in the vitality, attesting its antiproliferativeactivity (P < 0.05). The increased levels of the protein p-53 and the reduced levels of the proteins c-Jun and c-Fos athigher concentrations of ASTA, as well as, suggest the pro-apoptotic and anti-cancerous effects that this extract has on hepatocellularcarcinomas, confirmed also by caspase-3 activation. These findings suggest biotechnological utilisation of marine byproductsfor nutraceutical and pharmaceutical applications avoiding the employment of organic solvents for extraction.

UR - http://hdl.handle.net/10447/363691

UR - https://link.springer.com/article/10.1007/s10126-019-09904-y

M3 - Article

VL - 21

SP - 565

EP - 576

JO - Marine Biotechnology

JF - Marine Biotechnology

SN - 1436-2228

ER -