An extensive survey of wild Sicilian oregano was made. A total of 57 samples were collected fromvarious sites, followed by taxonomic characterization from an agronomic perspective. Based onmorphological and production characteristics obtained from the 57 samples, cluster analysis was used todivide the samples into homogeneous groups, to identify the best biotypes. All samples were analyzed fortheir phytochemical content, applying a cascade-extraction protocol and hydrodistillation, to obtain thenon volatile components and the essential oils, respectively. The extracts contained thirteen polyphenolderivatives, i.e., four flavanones, seven flavones, and two organic acids. Their qualitative and quantitativecharacterization was carried out by LC/MS analyses. The essential oils were characterized using acombination of GC-FID and GC/MS analyses; a total of 81 components were identified. The majorcomponents of the oils were thymol, p-cymene, and g-terpinene. Cluster analysis was carried out on bothphytochemical profiles and resulted in the division of the oregano samples into different chemical groups.The antioxidant activity of the essential oils and extracts was investigated by the Folin Ciocalteau (FC)colorimetric assay, by UV radiation-induced peroxidation in liposomal membranes (UV-IP test), and bydetermining the O. 2 -scavenging activity.
|Numero di pagine||23|
|Rivista||CHEMISTRY & BIODIVERSITY|
|Stato di pubblicazione||Published - 2013|