Petroleum hydrocarbons are the most widespread contaminants in the marineenvironment. The fate of petroleum in sea water is largely controlled by mechanical,physical and chemical factors which influencing the natural transformation of petroleum(oil weathering) and oil bio-degradation. On the basis of these considerations,bioremediation techniques have been developed and improved for cleaning up oil-pollutedmarine environment as alternative to chemical and physical techniques. To elucidate thecooperative action of mixed microbial populations in the biodegradation of crude oil, weconstructed artificial consortia composed of two to three bacteria (Alcanivoraxborkumensis strain SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeriSDM)capable to degrade oil hydrocarbons. Using these consortia, we investigated themechanism by which efficient biodegradation of crude oil could be accomplished by themixed populations for application in natural environment (bioaugmentation). Allexperiments were carried out in microcosms systems containing seawater (with andwithout inorganic nutrients) and oil was used as sole carbon source. All data obtained(Total DAPI Count, Live/Dead staining, Card-FISH and GC-FID hydrocarbons analysis)confirmed the fundamental role of bacteria belonging to Alcanivorax genus in degradationof linear hydrocarbons in oil polluted environments. Indeed, in all experimentationscarried out in seawater with or without inorganic nutrients showed as rates of oildegradation are almost total in presence of this bacterium.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2013|