TY - JOUR
T1 - Biocompatibility and biodistribution of functionalized carbon nano-onions (f-CNOs) in a vertebrate model
AU - Sancataldo, Giuseppe
AU - Giordani, Silvia
AU - Brescia, Rosaria
AU - Bartelmess, Juergen
AU - Rodio, Marina
AU - D'Amora, Marta
AU - Sancataldo, Giuseppe
AU - Cella Zanacchi, Francesca
AU - Diaspro, Alberto
PY - 2016
Y1 - 2016
N2 - Functionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake and low cytotoxicity. To date, the toxicological effects of f-CNOs on vertebrates have not been reported. In this study, the possible biological impact of f-CNOs on zebrafish during development is investigated, evaluating different toxicity end-points such as the survival rate, hatching rate, and heart beat rate. Furthermore, a bio-distribution study of boron dipyrromethene (BODIPY) functionalized CNOs in zebrafish larvae is performed by utilizing inverted selective plane illumination microscopy (iSPIM), due to its intrinsic capability of allowing for fast 3D imaging. Our in vivo findings indicate that f-CNOs exhibit no toxicity, good biocompatibility (in the concentration range of 5-100 μg mL-1) and a homogenous biodistribution in zebrafish larvae.
AB - Functionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake and low cytotoxicity. To date, the toxicological effects of f-CNOs on vertebrates have not been reported. In this study, the possible biological impact of f-CNOs on zebrafish during development is investigated, evaluating different toxicity end-points such as the survival rate, hatching rate, and heart beat rate. Furthermore, a bio-distribution study of boron dipyrromethene (BODIPY) functionalized CNOs in zebrafish larvae is performed by utilizing inverted selective plane illumination microscopy (iSPIM), due to its intrinsic capability of allowing for fast 3D imaging. Our in vivo findings indicate that f-CNOs exhibit no toxicity, good biocompatibility (in the concentration range of 5-100 μg mL-1) and a homogenous biodistribution in zebrafish larvae.
UR - http://hdl.handle.net/10447/468633
M3 - Article
SN - 2045-2322
VL - 6
SP - 33923-1-33923-9
JO - Scientific Reports
JF - Scientific Reports
ER -