TY - JOUR
T1 - BIOCOMPATIBILITY AND BIODEGRADABILITY OF ELECTROSPUN PHEA-PLA SCAFFOLDS: OUR PRELIMINARY EXPERIENCE IN A MURINE ANIMAL MODEL
AU - Damiano, Giuseppe
AU - Lo Monte, Attilio Ignazio
AU - Licciardi, Mariano
AU - Fiorica, Calogero
AU - Gioviale, Maria Concetta
AU - Abruzzo, Alida
AU - Sammartano, Antonino
AU - Pitarresi, Giovanna
AU - Cacciabaudo, Francesco
AU - Altomare, Roberta
AU - Genova, Pietro
AU - Buscemi, Giuseppe
AU - Giammona, Gaetano
AU - Palumbo, Vincenzo Davide
AU - Belmonte, Beatrice
AU - Tripodo, Claudio
AU - Palumbo, Fabio Salvatore
AU - Damiano, Giuseppe
AU - Bellavia, Maurizio
AU - Cassata, Giovanni
AU - Belmonte, null
AU - Fiorica, Calogero
AU - Licciardi, Mariano
AU - Spinelli, Gabriele
AU - Cacciabaudo, null
AU - Altomare, null
AU - Salina, null
AU - Genova, null
AU - Palumbo, Fabio S.
AU - Abbruzzo, Alida
AU - Abbruzzo, Alida
AU - Gioviale, Maria Concetta
AU - Tripodo, null
AU - Lo Monte, Attilio Ignazio
AU - Palumbo, Vincenzo Davide
AU - Buscemi, Giuseppe
AU - Pitarresi, Giovanna
AU - Giammona, null
AU - Sammartano, Antonino
AU - Spinelli, Gabriele
AU - Bellavia, Maurizio
PY - 2012
Y1 - 2012
N2 - We obtained a nano-fibrillar scaffold starting from a polymeric solution which, through electrospinning, gave a biodegradable material with optimal mechanical features and the capacity to allow cell adhesion. In this paper we report the in-vivo application on a murine animal model of two electrospun biodegradable materials, specifically designed to create tubular structures. In one case PHEA-PLA was co-spun with silk fibroin (Fibro-PHEAPLA) by a parallel electrospinning process to obtain a scaffold with two different polymeric fibers. In the other case, PHEA-PLA was mixed with polycaprolactone (PCLPHEA-PLA) to obtain a hybrid fibers scaffold. The in-vitro assay showed fibroblast colonization in both materials. The scaffolds were implanted in the dorsal fascial pouch of rats to evaluate their in-vivo Biocompatibility and tissue integration. Histopathological findings showed that after implantation a neutrophilic reaction associated to colliquative necrosis was predominant, particularly for PCL-PHEA-PLA. Fibro-PCL-PHEA caused a non organized stromal reaction. Cell adhesion was confirmed at SEM scan. Both materials were totally absorbed after 40 days with an inflammatory reaction. This preliminary studyshowed that biocompatibility of the scaffolds needs further investigation. The capability of the materials to be functionalized could allow us to modulate the inflammatory host response.
AB - We obtained a nano-fibrillar scaffold starting from a polymeric solution which, through electrospinning, gave a biodegradable material with optimal mechanical features and the capacity to allow cell adhesion. In this paper we report the in-vivo application on a murine animal model of two electrospun biodegradable materials, specifically designed to create tubular structures. In one case PHEA-PLA was co-spun with silk fibroin (Fibro-PHEAPLA) by a parallel electrospinning process to obtain a scaffold with two different polymeric fibers. In the other case, PHEA-PLA was mixed with polycaprolactone (PCLPHEA-PLA) to obtain a hybrid fibers scaffold. The in-vitro assay showed fibroblast colonization in both materials. The scaffolds were implanted in the dorsal fascial pouch of rats to evaluate their in-vivo Biocompatibility and tissue integration. Histopathological findings showed that after implantation a neutrophilic reaction associated to colliquative necrosis was predominant, particularly for PCL-PHEA-PLA. Fibro-PCL-PHEA caused a non organized stromal reaction. Cell adhesion was confirmed at SEM scan. Both materials were totally absorbed after 40 days with an inflammatory reaction. This preliminary studyshowed that biocompatibility of the scaffolds needs further investigation. The capability of the materials to be functionalized could allow us to modulate the inflammatory host response.
UR - http://hdl.handle.net/10447/71103
M3 - Article
VL - 7
SP - 841
EP - 851
JO - Digest Journal of Nanomaterials and Biostructures
JF - Digest Journal of Nanomaterials and Biostructures
SN - 1842-3582
ER -