BIOCOMPATIBILITY AND BIODEGRADABILITY OF ELECTROSPUN PHEA-PLA SCAFFOLDS: OUR PRELIMINARY EXPERIENCE IN A MURINE ANIMAL MODEL

Fabio Salvatore Palumbo, Alida Abruzzo, Attilio Ignazio Lo Monte, Francesco Cacciabaudo, Beatrice Belmonte, Giuseppe Buscemi, Vincenzo Davide Palumbo, Mariano Licciardi, Gaetano Giammona, Maria Concetta Gioviale, Antonino Sammartano, Calogero Fiorica, Pietro Genova, Roberta Altomare, Giuseppe Damiano, Claudio Tripodo, Giovanna Pitarresi, Giuseppe Damiano, Maurizio Bellavia, Giovanni CassataBelmonte, Calogero Fiorica, Mariano Licciardi, Gabriele Spinelli, Cacciabaudo, Altomare, Salina, Genova, Fabio S. Palumbo, Alida Abbruzzo, Alida Abbruzzo, Maria Concetta Gioviale, Tripodo, Attilio Ignazio Lo Monte, Vincenzo Davide Palumbo, Giuseppe Buscemi, Giovanna Pitarresi, Giammona, Antonino Sammartano, Gabriele Spinelli, Maurizio Bellavia

Risultato della ricerca: Article

5 Citazioni (Scopus)

Abstract

We obtained a nano-fibrillar scaffold starting from a polymeric solution which, through electrospinning, gave a biodegradable material with optimal mechanical features and the capacity to allow cell adhesion. In this paper we report the in-vivo application on a murine animal model of two electrospun biodegradable materials, specifically designed to create tubular structures. In one case PHEA-PLA was co-spun with silk fibroin (Fibro-PHEAPLA) by a parallel electrospinning process to obtain a scaffold with two different polymeric fibers. In the other case, PHEA-PLA was mixed with polycaprolactone (PCLPHEA-PLA) to obtain a hybrid fibers scaffold. The in-vitro assay showed fibroblast colonization in both materials. The scaffolds were implanted in the dorsal fascial pouch of rats to evaluate their in-vivo Biocompatibility and tissue integration. Histopathological findings showed that after implantation a neutrophilic reaction associated to colliquative necrosis was predominant, particularly for PCL-PHEA-PLA. Fibro-PCL-PHEA caused a non organized stromal reaction. Cell adhesion was confirmed at SEM scan. Both materials were totally absorbed after 40 days with an inflammatory reaction. This preliminary studyshowed that biocompatibility of the scaffolds needs further investigation. The capability of the materials to be functionalized could allow us to modulate the inflammatory host response.
Lingua originaleEnglish
pagine (da-a)841-851
Numero di pagine11
RivistaDigest Journal of Nanomaterials and Biostructures
Volume7
Stato di pubblicazionePublished - 2012

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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