Intestinal epithelial cells (IECs) play a pivotal role in maintaining intestinal homeostasis.Dierent noxious agents, among them also anticancer therapies, can impair intestinal epithelialintegrity triggering inflammation and oxidative stress. A frequent complication of chemotherapy isgastrointestinal mucositis, strongly influencing the eectiveness of therapy, increasing healthcare costs,and impairing patients’ quality of life. Dierent strategies are used to treat gastrointestinal mucositis,including products from natural sources. Our study focused on the eect of pomegranate (Punicagranatum L.) juice extract on IEC-6 cells, both during inflammatory conditions and following treatmentwith 5-fluorouracil (5-FU). The polyphenolic profile of pomegranate juice was characterized in detail byOnline Comprehensive two dimensional Liquid Chromatography-Mass Spectrometry. The evaluationof pomegranate juice extract in IEC-6 indicates a significant inhibition in proinflammatory factors,such as cytokines release, cyclooxygenase-2 and inducible nitric oxide synthase expression, andnitrotyrosine formation. Pomegranate also inhibited oxidative stress and adhesion protein expression.In 5-FU-treated IEC-6, pomegranate also inhibited both inflammatory and oxidative stress parametersand apoptosis. It promoted wound repair and tight junction expression. These results suggesta potential use of pomegranate as an adjuvant in the treatment of intestinal inflammatory andoxidative stress states, which also occur during chemotherapy-induced mucositis.
|Numero di pagine||20|
|Stato di pubblicazione||Published - 2020|
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