Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium

Guarneri, R.; Scondotto, S.

Risultato della ricerca: Article

2 Citazioni (Scopus)

Abstract

[3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. © 1990 Plenum Publishing Corporation.
Lingua originaleEnglish
pagine (da-a)773-776
Numero di pagine4
RivistaNeurochemical Research
Volume15
Stato di pubblicazionePublished - 1990

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Flunitrazepam
Neurons
Hormones
gamma-Aminobutyric Acid
Rats
Brain
Binding Sites
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium. / Guarneri, R.; Scondotto, S.

In: Neurochemical Research, Vol. 15, 1990, pag. 773-776.

Risultato della ricerca: Article

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abstract = "[3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. {\circledC} 1990 Plenum Publishing Corporation.",
keywords = "Flunitrazepam; GABA; neuronal culture; Biochemistry; Cellular and Molecular Neuroscience",
author = "{Guarneri, R.; Scondotto, S.} and Federico Piccoli and Giovanni Savettieri and Giuseppe Salemi and Donatella Ferraro and {La Bella}, Vincenzo",
year = "1990",
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pages = "773--776",
journal = "Neurochemical Research",
issn = "0364-3190",
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TY - JOUR

T1 - Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium

AU - Guarneri, R.; Scondotto, S.

AU - Piccoli, Federico

AU - Savettieri, Giovanni

AU - Salemi, Giuseppe

AU - Ferraro, Donatella

AU - La Bella, Vincenzo

PY - 1990

Y1 - 1990

N2 - [3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. © 1990 Plenum Publishing Corporation.

AB - [3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. © 1990 Plenum Publishing Corporation.

KW - Flunitrazepam; GABA; neuronal culture; Biochemistry; Cellular and Molecular Neuroscience

UR - http://hdl.handle.net/10447/207501

M3 - Article

VL - 15

SP - 773

EP - 776

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

ER -