To study the pathogen-host relationship, we used the model of the entomopathogenic bacterium Bacillus thuringiensis (Bt) and Rhynchophorus ferrugineus, a quarantine pest that attacks the palm trees. In particular, we focused on the Bt stress-induced infections. We studied the effect of Bt on larval growth, on hemocytes and on the expression of the heat shock proteins (Hsp70). HSPs are rapidly synthesized in the cells after a stress exposition including pathogens. The Hsp70 was evaluated in the supernatant of the hemocyte lysate (HLS) obtained from larvae fed with Bt. This is the first time that the presence of Hsp70 has been recorded in R. ferrugineus. Bt has negative effects on larval growth, on total hemocytes counts and on the hemocyte type. In addition Hsp70 expression was modulated in the time (3h, 6h, 12h, 24h) in the response to Bt ingestion, highlighting that Bt is a stress factor for the R. ferrugineus. Further investigation is needed to understand the possible correlation between the reduction of hemocytes and Hsp70 modulation.
|Numero di pagine||5|
|Stato di pubblicazione||Published - 2011|