Abstract

Autophagy is a highly regulated mechanism that enhances cell eukaryotic survival under various environmental and cellular stresses, by breakdown and recycling of macromolecules and organelles. Here we report that in Paracentrotus lividus embryos autophagic process occur, at a lesser extend during physiological development and at greater levels after a cadmium treatment. By Acridine Orange staining, we found that embryonic cells exposed to cadmium display green fluorescence in cytoplasm and nucleus, and show considerable red fluorescent dots in cytoplasm. This evidence suggests formation of acidic auto-phagolysosomal vacuoles. By Neutral Red vital staining, specific for lysosomes and cellular acid compartments, we obtained analogous results. These data have been sustained by anti-LC3 antibody detection (specific marker of autophagy). By Western blot we found a peak of physiological autophagy at late gastrula stage, while in treated embryos autophagy seem to anticipate massive apoptosis, probably for providing the energy for the removal of apoptotic cells or, alternately, as a less deleterious defence strategy for the safeguard of development program.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2009

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autophagy
Echinoidea
embryo (animal)
cadmium
cytoplasm
Paracentrotus lividus
acridine orange
lysosomes
antibody detection
automobiles
recycling
vacuoles
organelles
eukaryotic cells
apoptosis
cells
acids
energy

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title = "Autophagy during development of sea urchin embryos",
abstract = "Autophagy is a highly regulated mechanism that enhances cell eukaryotic survival under various environmental and cellular stresses, by breakdown and recycling of macromolecules and organelles. Here we report that in Paracentrotus lividus embryos autophagic process occur, at a lesser extend during physiological development and at greater levels after a cadmium treatment. By Acridine Orange staining, we found that embryonic cells exposed to cadmium display green fluorescence in cytoplasm and nucleus, and show considerable red fluorescent dots in cytoplasm. This evidence suggests formation of acidic auto-phagolysosomal vacuoles. By Neutral Red vital staining, specific for lysosomes and cellular acid compartments, we obtained analogous results. These data have been sustained by anti-LC3 antibody detection (specific marker of autophagy). By Western blot we found a peak of physiological autophagy at late gastrula stage, while in treated embryos autophagy seem to anticipate massive apoptosis, probably for providing the energy for the removal of apoptotic cells or, alternately, as a less deleterious defence strategy for the safeguard of development program.",
keywords = "Autofagy, sea urchin, development, cadmium",
author = "Roccheri, {Maria Carmela} and Roberto Chiarelli and Maria Agnello and Liana Bosco",
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TY - CONF

T1 - Autophagy during development of sea urchin embryos

AU - Roccheri, Maria Carmela

AU - Chiarelli, Roberto

AU - Agnello, Maria

AU - Bosco, Liana

PY - 2009

Y1 - 2009

N2 - Autophagy is a highly regulated mechanism that enhances cell eukaryotic survival under various environmental and cellular stresses, by breakdown and recycling of macromolecules and organelles. Here we report that in Paracentrotus lividus embryos autophagic process occur, at a lesser extend during physiological development and at greater levels after a cadmium treatment. By Acridine Orange staining, we found that embryonic cells exposed to cadmium display green fluorescence in cytoplasm and nucleus, and show considerable red fluorescent dots in cytoplasm. This evidence suggests formation of acidic auto-phagolysosomal vacuoles. By Neutral Red vital staining, specific for lysosomes and cellular acid compartments, we obtained analogous results. These data have been sustained by anti-LC3 antibody detection (specific marker of autophagy). By Western blot we found a peak of physiological autophagy at late gastrula stage, while in treated embryos autophagy seem to anticipate massive apoptosis, probably for providing the energy for the removal of apoptotic cells or, alternately, as a less deleterious defence strategy for the safeguard of development program.

AB - Autophagy is a highly regulated mechanism that enhances cell eukaryotic survival under various environmental and cellular stresses, by breakdown and recycling of macromolecules and organelles. Here we report that in Paracentrotus lividus embryos autophagic process occur, at a lesser extend during physiological development and at greater levels after a cadmium treatment. By Acridine Orange staining, we found that embryonic cells exposed to cadmium display green fluorescence in cytoplasm and nucleus, and show considerable red fluorescent dots in cytoplasm. This evidence suggests formation of acidic auto-phagolysosomal vacuoles. By Neutral Red vital staining, specific for lysosomes and cellular acid compartments, we obtained analogous results. These data have been sustained by anti-LC3 antibody detection (specific marker of autophagy). By Western blot we found a peak of physiological autophagy at late gastrula stage, while in treated embryos autophagy seem to anticipate massive apoptosis, probably for providing the energy for the removal of apoptotic cells or, alternately, as a less deleterious defence strategy for the safeguard of development program.

KW - Autofagy, sea urchin, development, cadmium

UR - http://hdl.handle.net/10447/40242

UR - http://fisv2009.azuleon.org/Poster_Session_I.php

M3 - Paper

ER -