Autophagy is a highly regulated mechanism that enhances cell eukaryotic survival under variousenvironmental and cellular stresses, by breakdown and recycling of macromolecules and organelles.Here we report that in Paracentrotus lividus embryos autophagic process occur, at a lesser extendduring physiological development and at greater levels after a cadmium treatment.By Acridine Orange staining, we found that embryonic cells exposed to cadmium display greenfluorescence in cytoplasm and nucleus, and show considerable red fluorescent dots in cytoplasm.This evidence suggests formation of acidic auto-phagolysosomal vacuoles. By Neutral Red vitalstaining, specific for lysosomes and cellular acid compartments, we obtained analogous results.These data have been sustained by anti-LC3 antibody detection (specific marker of autophagy). ByWestern blot we found a peak of physiological autophagy at late gastrula stage, while in treatedembryos autophagy seem to anticipate massive apoptosis, probably for providing the energy for theremoval of apoptotic cells or, alternately, as a less deleterious defence strategy for the safeguard ofdevelopment program.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2009|