Association Between Single Nucleotide Polymorphisms in the Cyclooxygenase-2, Tumor Necrosis Factor-a, and Vascular Endothelial Growth Factor-A Genes, and Susceptibility to Hepatocellular Carcinoma

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Abstract

Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-a (TNF-a) are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellular carcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms (SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore the proteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agents have entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-a, and VEGF-A genes in patients with HCC versus LC patients and a control group. The aim of this article was to verify the correlation between the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162 healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLPPCR) method. The SNPs analyzed were: 1195G>A of the COX-2 gene, 308G>A of the TNF-a gene, and +936C>T of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our results confirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC ( p¼0.039), suggesting that this SNP may predispose to the development of HCC.
Lingua originaleEnglish
pagine (da-a)193-196
Numero di pagine4
RivistaOMICS
Volume15
Stato di pubblicazionePublished - 2011

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vif Genes
Cyclooxygenase 2
Polymorphism
Vascular Endothelial Growth Factor A
Single Nucleotide Polymorphism
Hepatocellular Carcinoma
Nucleotides
Tumor Necrosis Factor-alpha
Genes
Liver Cirrhosis
Liver
Inflammation Mediators
Restriction Fragment Length Polymorphisms
Inpatients
Alleles
Clinical Trials
Statistical methods
Control Groups

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Biochemistry

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@article{105d0360136c45fa9169d3713a60ef8f,
title = "Association Between Single Nucleotide Polymorphisms in the Cyclooxygenase-2, Tumor Necrosis Factor-a, and Vascular Endothelial Growth Factor-A Genes, and Susceptibility to Hepatocellular Carcinoma",
abstract = "Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-a (TNF-a) are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellular carcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms (SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore the proteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agents have entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-a, and VEGF-A genes in patients with HCC versus LC patients and a control group. The aim of this article was to verify the correlation between the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162 healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLPPCR) method. The SNPs analyzed were: 1195G>A of the COX-2 gene, 308G>A of the TNF-a gene, and +936C>T of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our results confirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC ( p¼0.039), suggesting that this SNP may predispose to the development of HCC.",
keywords = "nucleotide polymorphisms, cyclooxygenase-2, tumor necrosis factor-α, vascular endothelial growth factor-A genes,hepatocellular carcinoma.",
author = "Giuseppe Montalto and Maurizio Soresi and Angela Terranova and Lydia Giannitrapani and Giacalone, {Antonio Mario} and Daniele Balasus",
year = "2011",
language = "English",
volume = "15",
pages = "193--196",
journal = "OMICS A Journal of Integrative Biology",
issn = "1536-2310",
publisher = "Mary Ann Liebert Inc.",

}

TY - JOUR

T1 - Association Between Single Nucleotide Polymorphisms in the Cyclooxygenase-2, Tumor Necrosis Factor-a, and Vascular Endothelial Growth Factor-A Genes, and Susceptibility to Hepatocellular Carcinoma

AU - Montalto, Giuseppe

AU - Soresi, Maurizio

AU - Terranova, Angela

AU - Giannitrapani, Lydia

AU - Giacalone, Antonio Mario

AU - Balasus, Daniele

PY - 2011

Y1 - 2011

N2 - Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-a (TNF-a) are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellular carcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms (SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore the proteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agents have entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-a, and VEGF-A genes in patients with HCC versus LC patients and a control group. The aim of this article was to verify the correlation between the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162 healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLPPCR) method. The SNPs analyzed were: 1195G>A of the COX-2 gene, 308G>A of the TNF-a gene, and +936C>T of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our results confirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC ( p¼0.039), suggesting that this SNP may predispose to the development of HCC.

AB - Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-a (TNF-a) are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellular carcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms (SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore the proteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agents have entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-a, and VEGF-A genes in patients with HCC versus LC patients and a control group. The aim of this article was to verify the correlation between the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162 healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLPPCR) method. The SNPs analyzed were: 1195G>A of the COX-2 gene, 308G>A of the TNF-a gene, and +936C>T of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our results confirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC ( p¼0.039), suggesting that this SNP may predispose to the development of HCC.

KW - nucleotide polymorphisms, cyclooxygenase-2, tumor necrosis factor-α, vascular endothelial growth factor-A genes,hepatocellular carcinoma.

UR - http://hdl.handle.net/10447/169871

UR - http://www.ncbi.nlm.nih.gov/pubmed/21319995

M3 - Article

VL - 15

SP - 193

EP - 196

JO - OMICS A Journal of Integrative Biology

JF - OMICS A Journal of Integrative Biology

SN - 1536-2310

ER -