Association Between Single Nucleotide Polymorphismsin the Cyclooxygenase-2, Tumor Necrosis Factor-a,and Vascular Endothelial Growth Factor-A Genes,and Susceptibility to Hepatocellular Carcinoma

Maurizio Soresi, Giuseppe Montalto, Daniele Balasus, Lydia Giannitrapani, Melchiorre Cervello, Daniele Balasus, Lorenzo Marasà, Angela Terranova, Antonio Mario Giacalone

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Abstract

Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-a (TNF-a)are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellularcarcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms(SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore theproteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agentshave entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-a, and VEGF-A genes inpatients with HCC versus LC patients and a control group. The aim of this article was to verify the correlationbetween the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLPPCR)method. The SNPs analyzed were: 1195G>A of the COX-2 gene, 308G>A of the TNF-a gene, and+936C>T of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our resultsconfirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC ( p¼0.039),suggesting that this SNP may predispose to the development of HCC.
Lingua originaleEnglish
pagine (da-a)193-196
Numero di pagine4
RivistaDefault journal
Volume15
Stato di pubblicazionePublished - 2011

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vif Genes
Cyclooxygenase 2
Polymorphism
Vascular Endothelial Growth Factor A
Single Nucleotide Polymorphism
Hepatocellular Carcinoma
Nucleotides
Tumor Necrosis Factor-alpha
Genes
Liver Cirrhosis
Liver
Inflammation Mediators
Restriction Fragment Length Polymorphisms
Inpatients
Alleles
Clinical Trials
Statistical methods
Control Groups

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics

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title = "Association Between Single Nucleotide Polymorphismsin the Cyclooxygenase-2, Tumor Necrosis Factor-a,and Vascular Endothelial Growth Factor-A Genes,and Susceptibility to Hepatocellular Carcinoma",
abstract = "Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-a (TNF-a)are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellularcarcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms(SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore theproteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agentshave entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-a, and VEGF-A genes inpatients with HCC versus LC patients and a control group. The aim of this article was to verify the correlationbetween the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLPPCR)method. The SNPs analyzed were: 1195G>A of the COX-2 gene, 308G>A of the TNF-a gene, and+936C>T of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our resultsconfirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC ( p¼0.039),suggesting that this SNP may predispose to the development of HCC.",
keywords = "cyclooxygenase-2, hepatocellular carcinoma., nucleotide polymorphisms, tumor necrosis factor-α, vascular endothelial growth factor-A genes",
author = "Maurizio Soresi and Giuseppe Montalto and Daniele Balasus and Lydia Giannitrapani and Melchiorre Cervello and Daniele Balasus and Lorenzo Maras{\`a} and Angela Terranova and Giacalone, {Antonio Mario}",
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TY - JOUR

T1 - Association Between Single Nucleotide Polymorphismsin the Cyclooxygenase-2, Tumor Necrosis Factor-a,and Vascular Endothelial Growth Factor-A Genes,and Susceptibility to Hepatocellular Carcinoma

AU - Soresi, Maurizio

AU - Montalto, Giuseppe

AU - Balasus, Daniele

AU - Giannitrapani, Lydia

AU - Cervello, Melchiorre

AU - Balasus, Daniele

AU - Marasà, Lorenzo

AU - Terranova, Angela

AU - Giacalone, Antonio Mario

PY - 2011

Y1 - 2011

N2 - Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-a (TNF-a)are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellularcarcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms(SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore theproteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agentshave entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-a, and VEGF-A genes inpatients with HCC versus LC patients and a control group. The aim of this article was to verify the correlationbetween the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLPPCR)method. The SNPs analyzed were: 1195G>A of the COX-2 gene, 308G>A of the TNF-a gene, and+936C>T of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our resultsconfirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC ( p¼0.039),suggesting that this SNP may predispose to the development of HCC.

AB - Cyclooxygenase-2 (COX-2), vascular endothelial growth factor-A (VEGF-A), and tumor necrosis factor-a (TNF-a)are mediators of inflammation and angiogenesis; all of them are produced in liver cirrhosis (LC) and in hepatocellularcarcinoma (HCC). It was proposed that there is an association between single nucleotide polymorphisms(SNPs) and HCC. These allelic variants influence the transcriptional activity of these genes, and therefore theproteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several antiangiogenic agentshave entered clinical trials in HCC. We evaluated the frequency of SNPs of COX-2, TNF-a, and VEGF-A genes inpatients with HCC versus LC patients and a control group. The aim of this article was to verify the correlationbetween the allelic variations and the risk of developing HCC. The study included 96 HCC, 79 LC patients, and 162healthy subjects. The evaluation of SNPs was performed by the restriction fragment length polymorphism (RFLPPCR)method. The SNPs analyzed were: 1195G>A of the COX-2 gene, 308G>A of the TNF-a gene, and+936C>T of the VEGF-A gene. Chi-square and Fisher exact tests were used for statistical analysis. Our resultsconfirm that carriers with the C allele in the VEGF-A gene are more frequent in HCC versus LC ( p¼0.039),suggesting that this SNP may predispose to the development of HCC.

KW - cyclooxygenase-2

KW - hepatocellular carcinoma.

KW - nucleotide polymorphisms

KW - tumor necrosis factor-α

KW - vascular endothelial growth factor-A genes

UR - http://hdl.handle.net/10447/169871

UR - http://www.ncbi.nlm.nih.gov/pubmed/21319995

M3 - Article

VL - 15

SP - 193

EP - 196

JO - Default journal

JF - Default journal

ER -