Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.

Manfredi Rizzo, Francesco Cappello, Francesco Carini, Luigi Nibali, Ricardo Munõz, Francisco Mesa, Navidah Chaudhary, Manfredi Rizzo, Francisco O'Valle, Francisco O'Valle, Francisco O'Valle, Nikos Donos, Mohamed Parkar, Francesco Cappello, Manfredi Rizzo

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12 Citazioni (Scopus)

Abstract

OBJECTIVES:The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.DESIGN:A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.RESULTS:The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).CONCLUSIONS:COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.
Lingua originaleEnglish
Numero di pagine6
RivistaPLoS One
Volume9
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Rizzo, M., Cappello, F., Carini, F., Nibali, L., Munõz, R., Mesa, F., Chaudhary, N., Rizzo, M., O'Valle, F., O'Valle, F., O'Valle, F., Donos, N., Parkar, M., Cappello, F., & Rizzo, M. (2014). Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study. PLoS One, 9.