Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.

Manfredi Rizzo, Francesco Cappello, Francesco Carini, Luigi Nibali, Ricardo Munõz, Francisco Mesa, Navidah Chaudhary, Manfredi Rizzo, Francisco O'Valle, Francisco O'Valle, Nikos Donos, Mohamed Parkar, Francesco Cappello, Manfredi Rizzo

Risultato della ricerca: Article

11 Citazioni (Scopus)

Abstract

OBJECTIVES:The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.DESIGN:A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.RESULTS:The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).CONCLUSIONS:COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.
Lingua originaleEnglish
Numero di pagine6
RivistaPLoS One
Volume9
Stato di pubblicazionePublished - 2014

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prostaglandin synthase
interleukin-6
connective tissues
Connective Tissue
Interleukin-6
Genotype
Tissue
genotype
Biopsy
Periodontal Diseases
laminae (animals)
Mucous Membrane
biopsy
Chronic Periodontitis
Gingivitis
gingivitis
cells
Haplotypes
case-control studies
Single Nucleotide Polymorphism

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cita questo

Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study. / Rizzo, Manfredi; Cappello, Francesco; Carini, Francesco; Nibali, Luigi; Munõz, Ricardo; Mesa, Francisco; Chaudhary, Navidah; Rizzo, Manfredi; O'Valle, Francisco; O'Valle, Francisco; Donos, Nikos; Parkar, Mohamed; Cappello, Francesco; Rizzo, Manfredi.

In: PLoS One, Vol. 9, 2014.

Risultato della ricerca: Article

Rizzo, M, Cappello, F, Carini, F, Nibali, L, Munõz, R, Mesa, F, Chaudhary, N, Rizzo, M, O'Valle, F, O'Valle, F, Donos, N, Parkar, M, Cappello, F & Rizzo, M 2014, 'Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.', PLoS One, vol. 9.
Rizzo, Manfredi ; Cappello, Francesco ; Carini, Francesco ; Nibali, Luigi ; Munõz, Ricardo ; Mesa, Francisco ; Chaudhary, Navidah ; Rizzo, Manfredi ; O'Valle, Francisco ; O'Valle, Francisco ; Donos, Nikos ; Parkar, Mohamed ; Cappello, Francesco ; Rizzo, Manfredi. / Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study. In: PLoS One. 2014 ; Vol. 9.
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title = "Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.",
abstract = "OBJECTIVES:The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.DESIGN:A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.RESULTS:The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).CONCLUSIONS:COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.",
author = "Manfredi Rizzo and Francesco Cappello and Francesco Carini and Luigi Nibali and Ricardo Mun{\~o}z and Francisco Mesa and Navidah Chaudhary and Manfredi Rizzo and Francisco O'Valle and Francisco O'Valle and Nikos Donos and Mohamed Parkar and Francesco Cappello and Manfredi Rizzo",
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TY - JOUR

T1 - Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.

AU - Rizzo, Manfredi

AU - Cappello, Francesco

AU - Carini, Francesco

AU - Nibali, Luigi

AU - Munõz, Ricardo

AU - Mesa, Francisco

AU - Chaudhary, Navidah

AU - Rizzo, Manfredi

AU - O'Valle, Francisco

AU - O'Valle, Francisco

AU - Donos, Nikos

AU - Parkar, Mohamed

AU - Cappello, Francesco

AU - Rizzo, Manfredi

PY - 2014

Y1 - 2014

N2 - OBJECTIVES:The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.DESIGN:A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.RESULTS:The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).CONCLUSIONS:COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.

AB - OBJECTIVES:The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.DESIGN:A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.RESULTS:The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).CONCLUSIONS:COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.

UR - http://hdl.handle.net/10447/92249

UR - http://www.ncbi.nlm.nih.gov/pubmed/24551049

M3 - Article

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

ER -