Artificial chromosome libraries of Streptomyces coelicolor A3(2) and Planobispora rosea

Simona De Grazia, Anna Maria Puglia, Rosa Alduina, Margherita Sosio, Anna M. Puglia, Stefano Donadio

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13 Citazioni (Scopus)

Abstract

Using an Escherichia coli-Streptomyces shuttle vector derived from a bacterial artificial chromosome (BAC), we developed methodologies for the construction of BAC libraries of filamentous actinomycetes. Libraries of Streptomyces coelicolor, the model actinomycete, and Planobispora rosea, a genetically intractable strain, were constructed. Both libraries have an average insert size of 60 kb, with maximal insert larger than 150 kb. The S. coelicolor library was evaluated by selected hybridisations to DraI fragments and by end sequencing of a few clones. Hybridisation of the P. rosea library to selected probes indicates a good representation of the P. rosea genome and that the library can be used to facilitate the genomic analysis of this actinomycete.
Lingua originaleEnglish
pagine (da-a)181-186
Numero di pagine6
RivistaFEMS Microbiology Letters
Volume218
Stato di pubblicazionePublished - 2003

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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