Thyroid cancer is the most frequent endocrine malignancy with a global increasingincidence. Many evidences show that sulphoraphane (SF), a natural isothiocyante found incruciferous vegetables has a wide range of chemopreventive as well as apoptosis inducingproperties. The ability of SF in inducing apoptosis and cell cycle arrest is associated withthe regulation of many proteins including Bcl-2 family proteins, caspases, p21, and cyclindependent kinases.In the present work, we investigated in vitro the activity of SF in three human thyroidcancer cell lines. For this purpose we studied SW1736 (ATC), BC-PAP (PTC) and TT(MTC) cell line by MTT assay, after addition of SF ranging from 0 to 20M. Cell lineswere treated with SF at different concentration (1M, 5M, 10M, 20M) for 72 hours andafter treatment we observed a SF- induced toxicity in all thyroid cancer cell lines, whereasSF had not significant effects on nonmalignant cells. In order to demonstrate the role of SFin the apoptotic pathway, we analyzed its effect on Bcl-2 and BAX protein expression bywestern blot. The increase of SF concentration induce an upregulation of BAX followed bydown-regulation of Bcl-2, confirming its pro-apoptotic role in all three cell lines. So, ourpreliminary data, suggest its possible use in prevention trial in high-risk areas and asenhancer in anti-neoplastic therapy of thyroid cancer.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2013|