Antiendomysium antibodies assay in the culture medium of intestinal mucosa: an accurate method for celiac diseasediagnosis

Gaetana Di Fede, Ilenia Pepe, Ada Maria Florena, Giuseppina Campisi, Carmelo Sciume', Girolamo Geraci, Antonio Carroccio, Pasquale Mansueto, Maurizio Soresi, Saverio Teresi, Antonio Carroccio, Francesca Cavataio, Giuseppe Iacono, Giuseppe Ambrosiano, Giuseppe Pirrone, Lidia Di Prima

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Abstract

Background Celiac disease (CD) diagnosis is becoming more difficult as patients with no intestinal histology lesions may also be suffering from CD. Aim To evaluate the diagnostic accuracy of antiendomysium (EmA) assay in the culture medium of intestinal biopsies for CD diagnosis. Patients and methods The clinical charts of 418 patients with CD and 705 non-CD controls who had all undergone EmA assay in the culture medium were reviewed. Results EmA assay in the culture medium had a higher sensitivity (98 vs. 80%) and specificity (99 vs. 95%) than serum EmA/antibodies to tissue transglutaminase (anti-tTG) assay. All patients with CD who were tested as false-negatives for serum EmA and/or anti-tTG (32 adults and 39 children) carried the human leukocyte antigen alleles associated to CD. Furthermore, during the follow-up, four patients with negative-serum EmA/anti-tTG, normal villi architecture, and positive-EmAs in the culture medium, developed villous atrophy and underwent gluten-free diet with consequent resolution of the symptoms and complete intestinal histology recovery. Conclusion EmA assay in the culture medium should be included in the diagnostic criteria for CD diagnosis in 'seronegative' patients. Eur J Gastroenterol Hepatol 23:1018-1023 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
Lingua originaleEnglish
pagine (da-a)1018-1023
Numero di pagine6
RivistaEUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
Volume23
Stato di pubblicazionePublished - 2011

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Celiac Disease
Intestinal Mucosa
Abdomen
Culture Media
Antibodies
Histology
Serum
Gluten-Free Diet
HLA Antigens
Atrophy
Alleles
Biopsy
Health
transglutaminase 2

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

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@article{8b2d1458b507453b9d56bd743edfe1c3,
title = "Antiendomysium antibodies assay in the culture medium of intestinal mucosa: an accurate method for celiac diseasediagnosis",
abstract = "Background Celiac disease (CD) diagnosis is becoming more difficult as patients with no intestinal histology lesions may also be suffering from CD. Aim To evaluate the diagnostic accuracy of antiendomysium (EmA) assay in the culture medium of intestinal biopsies for CD diagnosis. Patients and methods The clinical charts of 418 patients with CD and 705 non-CD controls who had all undergone EmA assay in the culture medium were reviewed. Results EmA assay in the culture medium had a higher sensitivity (98 vs. 80{\%}) and specificity (99 vs. 95{\%}) than serum EmA/antibodies to tissue transglutaminase (anti-tTG) assay. All patients with CD who were tested as false-negatives for serum EmA and/or anti-tTG (32 adults and 39 children) carried the human leukocyte antigen alleles associated to CD. Furthermore, during the follow-up, four patients with negative-serum EmA/anti-tTG, normal villi architecture, and positive-EmAs in the culture medium, developed villous atrophy and underwent gluten-free diet with consequent resolution of the symptoms and complete intestinal histology recovery. Conclusion EmA assay in the culture medium should be included in the diagnostic criteria for CD diagnosis in 'seronegative' patients. Eur J Gastroenterol Hepatol 23:1018-1023 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.",
author = "{Di Fede}, Gaetana and Ilenia Pepe and Florena, {Ada Maria} and Giuseppina Campisi and Carmelo Sciume' and Girolamo Geraci and Antonio Carroccio and Pasquale Mansueto and Maurizio Soresi and Saverio Teresi and Antonio Carroccio and Francesca Cavataio and Giuseppe Iacono and Giuseppe Ambrosiano and Giuseppe Pirrone and {Di Prima}, Lidia",
year = "2011",
language = "English",
volume = "23",
pages = "1018--1023",
journal = "EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY",
issn = "0954-691X",

}

TY - JOUR

T1 - Antiendomysium antibodies assay in the culture medium of intestinal mucosa: an accurate method for celiac diseasediagnosis

AU - Di Fede, Gaetana

AU - Pepe, Ilenia

AU - Florena, Ada Maria

AU - Campisi, Giuseppina

AU - Sciume', Carmelo

AU - Geraci, Girolamo

AU - Carroccio, Antonio

AU - Mansueto, Pasquale

AU - Soresi, Maurizio

AU - Teresi, Saverio

AU - Carroccio, Antonio

AU - Cavataio, Francesca

AU - Iacono, Giuseppe

AU - Ambrosiano, Giuseppe

AU - Pirrone, Giuseppe

AU - Di Prima, Lidia

PY - 2011

Y1 - 2011

N2 - Background Celiac disease (CD) diagnosis is becoming more difficult as patients with no intestinal histology lesions may also be suffering from CD. Aim To evaluate the diagnostic accuracy of antiendomysium (EmA) assay in the culture medium of intestinal biopsies for CD diagnosis. Patients and methods The clinical charts of 418 patients with CD and 705 non-CD controls who had all undergone EmA assay in the culture medium were reviewed. Results EmA assay in the culture medium had a higher sensitivity (98 vs. 80%) and specificity (99 vs. 95%) than serum EmA/antibodies to tissue transglutaminase (anti-tTG) assay. All patients with CD who were tested as false-negatives for serum EmA and/or anti-tTG (32 adults and 39 children) carried the human leukocyte antigen alleles associated to CD. Furthermore, during the follow-up, four patients with negative-serum EmA/anti-tTG, normal villi architecture, and positive-EmAs in the culture medium, developed villous atrophy and underwent gluten-free diet with consequent resolution of the symptoms and complete intestinal histology recovery. Conclusion EmA assay in the culture medium should be included in the diagnostic criteria for CD diagnosis in 'seronegative' patients. Eur J Gastroenterol Hepatol 23:1018-1023 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

AB - Background Celiac disease (CD) diagnosis is becoming more difficult as patients with no intestinal histology lesions may also be suffering from CD. Aim To evaluate the diagnostic accuracy of antiendomysium (EmA) assay in the culture medium of intestinal biopsies for CD diagnosis. Patients and methods The clinical charts of 418 patients with CD and 705 non-CD controls who had all undergone EmA assay in the culture medium were reviewed. Results EmA assay in the culture medium had a higher sensitivity (98 vs. 80%) and specificity (99 vs. 95%) than serum EmA/antibodies to tissue transglutaminase (anti-tTG) assay. All patients with CD who were tested as false-negatives for serum EmA and/or anti-tTG (32 adults and 39 children) carried the human leukocyte antigen alleles associated to CD. Furthermore, during the follow-up, four patients with negative-serum EmA/anti-tTG, normal villi architecture, and positive-EmAs in the culture medium, developed villous atrophy and underwent gluten-free diet with consequent resolution of the symptoms and complete intestinal histology recovery. Conclusion EmA assay in the culture medium should be included in the diagnostic criteria for CD diagnosis in 'seronegative' patients. Eur J Gastroenterol Hepatol 23:1018-1023 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

UR - http://hdl.handle.net/10447/73320

M3 - Article

VL - 23

SP - 1018

EP - 1023

JO - EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY

JF - EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY

SN - 0954-691X

ER -