Antibody-Fc/FcR interaction on macrophages as a mechanism for hyperprogressive disease in non-small cell lung cancer subsequent to PD-1/PD-L1 blockade

Claudio Tripodo, Valeria Cancila, Marina Chiara Garassino, Patrizia Gasparini, Michele Sommariva, Monica Ganzinelli, Luca Porcu, Massimo Moro, Diego Signorelli, Giovanni Centonze, Elena Tassi, Giuseppe Lo Russo, Chiara Storti, Claudia Proto, Andrea Anichini, Giancarlo Pruneri, Silvia Marsoni, Simona Ferro, Mattia Boeri, Sabina SangalettiLucia Sfondrini, Veronica Huber, Giancarlo Pruneri, Silvia Marsoni, Gabriella Sozzi, Licia Rivoltini, Andrea Balsari, Andrea Anichini, Valter Torri, Alberto Bardelli, Mario Paolo Colombo, Massimo Milione

Risultato della ricerca: Articlepeer review

148 Citazioni (Scopus)

Abstract

Purpose: Hyperprogression (HP), a paradoxical boost in tumor growth, was described in a subset of patients treated with immune checkpoint inhibitors (ICI). Neither clinico-pathologic features nor biological mechanisms associated with HP have been identified. Experimental Design: Among 187 patients with non-small cell lung cancer (NSCLC) treated with ICI at our institute, cases with HP were identified according to clinical and radiologic criteria. Baseline histologic samples from patients treated with ICI were evaluated by IHC for myeloid and lymphoid markers. T-cell-deficient mice, injected with human lung cancer cells and patient-derived xenografts (PDX) belonging to specific mutational subsets, were assessed for tumor growth after treatment with antibodies against mouse and human programmed death receptor-1 (PD-1). The immune microenvironment was evaluated by flow cytometry and IHC. Results: Among 187 patients, 152 were evaluable for clinical response. We identified four categories: 32 cases were defined as responders (21%), 42 patients with stable disease (27.7%), 39 cases were defined as progressors (25.7%), and 39 patients with HP (25.7%). Pretreatment tissue samples from all patients with HP showed tumor infiltration by M2-like CD163 þ CD33 þ PD-L1 þ clustered epithelioid macrophages. Enrichment by tumor-associated macrophages (TAM) was observed, even in tumor nodules from immunodeficient mice injected with human lung cancer cells and with PDXs. In these models, tumor growth was enhanced by treatment with anti-PD-1 but not anti-PD-1 F(ab) 2 fragments. Conclusions: These results suggest a crucial role of TAM reprogramming, upon Fc receptor engagement by ICI, eventually inducing HP and provide clues on a distinctive immunophenotype potentially able to predict HP.
Lingua originaleEnglish
pagine (da-a)989-999
Numero di pagine11
RivistaClinical Cancer Research
Volume25
Stato di pubblicazionePublished - 2019

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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