Anti-proliferative effect of main dietaryphytosterols and β-cryptoxanthin alone orcombined in human colon cancer Caco-2 cellsthrough cytosolic Ca+2 – and oxidative stress induced apoptosis

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Abstract

β-cryptoxanthin (β-Cx) and phytosterols (Ps) have potential against different cancer types,including colon cancer. However, their combined action has not been reported so far. Human colon cancer Caco-2 cells were treated 24 h with β-Cx and/or main dietary Ps (β-sitosterol, campesterol and stigmasterol), alone or in combination, at concentrations compatible withphysiological human serum levels. A decrease in cell viability due to apoptosis (rise in sub-G1 population and exposure of membrane phosphatidylserine) was accompanied withdephosphorylation of BAD, mitochondrial depolarization and caspase 3-dependent PARP cleavage,with intracellular Ca2+ influx and increase of RONS levels as initial triggers. Ps and β-Cx, alone or in combination showed anti-proliferative activity against human colon adenocarcinoma Caco-2 cells through the mitochondrial pathway of apoptosis. No additive or synergistic effects were observed.The importance of bioactivity-guided assays with mixtures of dietarybioactive compounds to determine their eventual interactions in the functional food context is demonstrated.
Lingua originaleEnglish
pagine (da-a)282-293
Numero di pagine12
RivistaJournal of Functional Foods
Volume12
Stato di pubblicazionePublished - 2015

All Science Journal Classification (ASJC) codes

  • ???subjectarea.asjc.1100.1106???
  • ???subjectarea.asjc.2700.2701???
  • ???subjectarea.asjc.2900.2916???

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