Abstract

Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity*. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) san-rples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsilile of the fluoropyrimidines drug resistance and the worse prognosis.
Lingua originaleEnglish
Numero di pagine0
RivistaDefault journal
Volume10
Stato di pubblicazionePublished - 2010

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Thymidylate Synthase
Fluorouracil
Genes
Pharmaceutical Preparations
Neoplasms
Drug Resistance
Colorectal Neoplasms
Methylation
Genomic Instability
DNA
Tumor Cell Line
Tumors
Cells
Mutation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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title = "Analysis of the Thymidylate Synthase Gene Structure inColorectal Cancer Patients and lts Possible Relationwith the S-Fluorouracil Drug Response",
abstract = "Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity*. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) san-rples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsilile of the fluoropyrimidines drug resistance and the worse prognosis.",
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T1 - Analysis of the Thymidylate Synthase Gene Structure inColorectal Cancer Patients and lts Possible Relationwith the S-Fluorouracil Drug Response

AU - Feo, Salvatore

AU - Cajozzo, Massimo

AU - Sanguedolce, Rosario

AU - Calascibetta, Anna

AU - Gulotta, Gaspare

AU - Contino, Flavia

PY - 2010

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N2 - Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity*. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) san-rples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsilile of the fluoropyrimidines drug resistance and the worse prognosis.

AB - Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity*. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) san-rples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsilile of the fluoropyrimidines drug resistance and the worse prognosis.

UR - http://hdl.handle.net/10447/96237

M3 - Article

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