Abstract

Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) samples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes'' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsible of the fluoropyrimidines drug resistance and the worse prognosis.
Lingua originaleEnglish
Numero di pagine0
RivistaDefault journal
Volume2009
Stato di pubblicazionePublished - 2009

Fingerprint

Thymidylate Synthase
Fluorouracil
Colorectal Neoplasms
Genes
Pharmaceutical Preparations
Drug Resistance
Methylation
Genomic Instability
DNA
Tumor Cell Line
Tumors
Cells
Mutation

Cita questo

@article{99af90c64b00430b999187d32c5afb34,
title = "Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients, and Its Possible Relation with the 5-Fluorouracil Drug Response",
abstract = "Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) samples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes'' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsible of the fluoropyrimidines drug resistance and the worse prognosis.",
keywords = "5-Fluorouracil, Colorectal Cancer, Drug Response",
author = "Salvatore Feo and Flavia Contino and Rosario Sanguedolce and Gaspare Gulotta and Anna Calascibetta and Angelo Antona and Massimo Cajozzo and Giorgio Sanguedolce",
year = "2009",
language = "English",
volume = "2009",
journal = "Default journal",

}

TY - JOUR

T1 - Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients, and Its Possible Relation with the 5-Fluorouracil Drug Response

AU - Feo, Salvatore

AU - Contino, Flavia

AU - Sanguedolce, Rosario

AU - Gulotta, Gaspare

AU - Calascibetta, Anna

AU - Antona, Angelo

AU - Cajozzo, Massimo

AU - Sanguedolce, Giorgio

PY - 2009

Y1 - 2009

N2 - Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) samples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes'' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsible of the fluoropyrimidines drug resistance and the worse prognosis.

AB - Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) samples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes'' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsible of the fluoropyrimidines drug resistance and the worse prognosis.

KW - 5-Fluorouracil

KW - Colorectal Cancer

KW - Drug Response

UR - http://hdl.handle.net/10447/41505

M3 - Article

VL - 2009

JO - Default journal

JF - Default journal

ER -