Analysis of seroprevalence against Coxiella burnetii in a sample of farm workers in Western Sicily

Paola De Marchis, Diego Picciotto, Domenica Matranga, Maria Gabriella Verso, Sara Villari, Paola Galluzzo, Valeria Gargano, Gesualdo Vesco

Risultato della ricerca: Article

5 Citazioni (Scopus)

Abstract

Introduction and objective. Little is known about the development of chronic Q fever caused by Coxiella burnetii in occupational risk groups and in the general population in Italy, as well as in many countries in the world. The aim of thisstudy was to highlight the presence of the infection in a sample of workers operating outdoors (but not directly in contact with animals), in three provinces of western Sicily, in order to detect the human seroprevalence and compare the obtained data with those found in animals raised in the same territory.Materials and methods. The study included 126 generic seasonal agricultural workers (labourers), 84 male and 42 female; none of whom were aware of any previous contact with Coxiella burnetii. Their immunologic status against Coxiella burnetii was tested through research and titration of both phases I and II specific antibodies (IgG) with an indirect immunofluorescence assay, using anti-antibodies labeled with fluorescein isothiocyanate. All data were statistically analyzed, comparing all positive results from the three provinces through variance analysis, and then comparing human results with those obtained fromanimals raised in the considered areas, specifically, 1,511 cows, 46 of which were found positive (3.04%), and 3,391 sheep, 548 of which were found positive (16.16%). Results. Anti-Coxiella antibodies were found in nine of 42 females sampled (21.4%; 95%CI=[9.0–33.8]) and 21 of 84 malessampled (25.0%; 95%CI=[15.7–34.3]). 60% (18 of 30; 95%CI=[42.5–77.5]) of seropositive samples were positive either for Phase I antigen or for both Phase I and II antigens, representing cases of chronic infection. Applying Spearman’s rank correlation, the percentage of seropositive humans was significantly correlated with that observed for sheep (r=1.00; p<0.001), but notfor cows (r=-0.5; p=0.667).Conclusions. The results obtained, although based on a small sample, suggest that the disease is present in the territory of Western Sicily, both in animals and in humans. A closer collaboration between doctors and veterinarians is thereforenecessary to fight against the spread of the infection.
Lingua originaleEnglish
pagine (da-a)71-74
Numero di pagine4
RivistaDefault journal
Volume23, N. 1
Stato di pubblicazionePublished - 2016

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Sicily
Coxiella burnetii
agricultural worker
farm labor
Seroepidemiologic Studies
seroprevalence
antibody
antigen
sheep
animal
antibodies
Anti-Idiotypic Antibodies
Sheep
Coxiella
Infection
infection
antigens
Q fever
Q Fever
cows

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Waste Management and Disposal
  • Public Health, Environmental and Occupational Health

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Analysis of seroprevalence against Coxiella burnetii in a sample of farm workers in Western Sicily. / De Marchis, Paola; Picciotto, Diego; Matranga, Domenica; Verso, Maria Gabriella; Villari, Sara; Galluzzo, Paola; Gargano, Valeria; Vesco, Gesualdo.

In: Default journal, Vol. 23, N. 1, 2016, pag. 71-74.

Risultato della ricerca: Article

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title = "Analysis of seroprevalence against Coxiella burnetii in a sample of farm workers in Western Sicily",
abstract = "Introduction and objective. Little is known about the development of chronic Q fever caused by Coxiella burnetii in occupational risk groups and in the general population in Italy, as well as in many countries in the world. The aim of thisstudy was to highlight the presence of the infection in a sample of workers operating outdoors (but not directly in contact with animals), in three provinces of western Sicily, in order to detect the human seroprevalence and compare the obtained data with those found in animals raised in the same territory.Materials and methods. The study included 126 generic seasonal agricultural workers (labourers), 84 male and 42 female; none of whom were aware of any previous contact with Coxiella burnetii. Their immunologic status against Coxiella burnetii was tested through research and titration of both phases I and II specific antibodies (IgG) with an indirect immunofluorescence assay, using anti-antibodies labeled with fluorescein isothiocyanate. All data were statistically analyzed, comparing all positive results from the three provinces through variance analysis, and then comparing human results with those obtained fromanimals raised in the considered areas, specifically, 1,511 cows, 46 of which were found positive (3.04{\%}), and 3,391 sheep, 548 of which were found positive (16.16{\%}). Results. Anti-Coxiella antibodies were found in nine of 42 females sampled (21.4{\%}; 95{\%}CI=[9.0–33.8]) and 21 of 84 malessampled (25.0{\%}; 95{\%}CI=[15.7–34.3]). 60{\%} (18 of 30; 95{\%}CI=[42.5–77.5]) of seropositive samples were positive either for Phase I antigen or for both Phase I and II antigens, representing cases of chronic infection. Applying Spearman’s rank correlation, the percentage of seropositive humans was significantly correlated with that observed for sheep (r=1.00; p<0.001), but notfor cows (r=-0.5; p=0.667).Conclusions. The results obtained, although based on a small sample, suggest that the disease is present in the territory of Western Sicily, both in animals and in humans. A closer collaboration between doctors and veterinarians is thereforenecessary to fight against the spread of the infection.",
author = "{De Marchis}, Paola and Diego Picciotto and Domenica Matranga and Verso, {Maria Gabriella} and Sara Villari and Paola Galluzzo and Valeria Gargano and Gesualdo Vesco",
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volume = "23, N. 1",
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TY - JOUR

T1 - Analysis of seroprevalence against Coxiella burnetii in a sample of farm workers in Western Sicily

AU - De Marchis, Paola

AU - Picciotto, Diego

AU - Matranga, Domenica

AU - Verso, Maria Gabriella

AU - Villari, Sara

AU - Galluzzo, Paola

AU - Gargano, Valeria

AU - Vesco, Gesualdo

PY - 2016

Y1 - 2016

N2 - Introduction and objective. Little is known about the development of chronic Q fever caused by Coxiella burnetii in occupational risk groups and in the general population in Italy, as well as in many countries in the world. The aim of thisstudy was to highlight the presence of the infection in a sample of workers operating outdoors (but not directly in contact with animals), in three provinces of western Sicily, in order to detect the human seroprevalence and compare the obtained data with those found in animals raised in the same territory.Materials and methods. The study included 126 generic seasonal agricultural workers (labourers), 84 male and 42 female; none of whom were aware of any previous contact with Coxiella burnetii. Their immunologic status against Coxiella burnetii was tested through research and titration of both phases I and II specific antibodies (IgG) with an indirect immunofluorescence assay, using anti-antibodies labeled with fluorescein isothiocyanate. All data were statistically analyzed, comparing all positive results from the three provinces through variance analysis, and then comparing human results with those obtained fromanimals raised in the considered areas, specifically, 1,511 cows, 46 of which were found positive (3.04%), and 3,391 sheep, 548 of which were found positive (16.16%). Results. Anti-Coxiella antibodies were found in nine of 42 females sampled (21.4%; 95%CI=[9.0–33.8]) and 21 of 84 malessampled (25.0%; 95%CI=[15.7–34.3]). 60% (18 of 30; 95%CI=[42.5–77.5]) of seropositive samples were positive either for Phase I antigen or for both Phase I and II antigens, representing cases of chronic infection. Applying Spearman’s rank correlation, the percentage of seropositive humans was significantly correlated with that observed for sheep (r=1.00; p<0.001), but notfor cows (r=-0.5; p=0.667).Conclusions. The results obtained, although based on a small sample, suggest that the disease is present in the territory of Western Sicily, both in animals and in humans. A closer collaboration between doctors and veterinarians is thereforenecessary to fight against the spread of the infection.

AB - Introduction and objective. Little is known about the development of chronic Q fever caused by Coxiella burnetii in occupational risk groups and in the general population in Italy, as well as in many countries in the world. The aim of thisstudy was to highlight the presence of the infection in a sample of workers operating outdoors (but not directly in contact with animals), in three provinces of western Sicily, in order to detect the human seroprevalence and compare the obtained data with those found in animals raised in the same territory.Materials and methods. The study included 126 generic seasonal agricultural workers (labourers), 84 male and 42 female; none of whom were aware of any previous contact with Coxiella burnetii. Their immunologic status against Coxiella burnetii was tested through research and titration of both phases I and II specific antibodies (IgG) with an indirect immunofluorescence assay, using anti-antibodies labeled with fluorescein isothiocyanate. All data were statistically analyzed, comparing all positive results from the three provinces through variance analysis, and then comparing human results with those obtained fromanimals raised in the considered areas, specifically, 1,511 cows, 46 of which were found positive (3.04%), and 3,391 sheep, 548 of which were found positive (16.16%). Results. Anti-Coxiella antibodies were found in nine of 42 females sampled (21.4%; 95%CI=[9.0–33.8]) and 21 of 84 malessampled (25.0%; 95%CI=[15.7–34.3]). 60% (18 of 30; 95%CI=[42.5–77.5]) of seropositive samples were positive either for Phase I antigen or for both Phase I and II antigens, representing cases of chronic infection. Applying Spearman’s rank correlation, the percentage of seropositive humans was significantly correlated with that observed for sheep (r=1.00; p<0.001), but notfor cows (r=-0.5; p=0.667).Conclusions. The results obtained, although based on a small sample, suggest that the disease is present in the territory of Western Sicily, both in animals and in humans. A closer collaboration between doctors and veterinarians is thereforenecessary to fight against the spread of the infection.

UR - http://hdl.handle.net/10447/174012

M3 - Article

VL - 23, N. 1

SP - 71

EP - 74

JO - Default journal

JF - Default journal

ER -