TY - CONF
T1 - ANALYSIS OF MICROSATELLITE MARKERS IN SICILIAN GOATS BREED FOR TRACEABILITY OF GIRGENTANATYPICAL DAIRY PRODUCTS
AU - Tortorici, Lina
AU - Sardina, Maria Teresa
AU - Tolone, Marco
AU - Di Gerlando, Rosalia
AU - Portolano, Baldassare
PY - 2013
Y1 - 2013
N2 - The establishment of useful analytical methods able to ensure the origin of the products, including thebreed used, are important in maintaining the reliability of these products in order to develop a marketsegment. Traceability, obtained by molecular analysis, could be a reliable proposal for the authenticationand valorization of animal products. In Sicily, the three most important dairy goat breeds are Girgentana(GR), Maltese (ML) and Derivata di Siria (DS). The GR is an endangered autochthonous goat breed.Preservation of endangered breeds could be achieved by establishing economic reasons for their survival.The aim of this work was to verify the use of microsatellite markers to assess a genetic traceability systemto discriminate breeds and to detect adulteration in GR dairy products.A total of 314 individuals belonging to GR, ML, and DS goat breeds were genotyped at 24 microsatellitemarkers and a subset of 9 microsatellites was chosen for further analysis due to the presence of privatealleles. The analyzed markers were FBC48, FBC20, CSRD247, SRCRSP5, OLADRB, SRCRSP8, OARAE54,SRCRSP24, and TGLA122. Fragments analysis of multiplex PCR was performed by capillary electrophoresiswith ABI3130xl Genetic Analyzer. Allele frequency, average number of alleles, allelic richness (AR), Ho andHe, PIC, and HWE for 9 loci were estimated using CERVUS 3.0.3 (Marshall et al., 1998), GENETIX (Belkhir etal., 1996), FSTAT 2.9.3.2 (Goudet, 1995) and POPGENE 1.31 (Yeh et al., 1999) software.The results showed high variability of markers subset considering that mean PIC values was 0.6449 andaverage number of alleles per locus was 8.67; AR ranged from 4.585 in TGLA122 to 8.980 in OLADRB. Sixmicrosatellite markers from subset were not in HWE probably due to heterozygote excess (Ho=0.6133).Intotal 4 private alleles were found in GR, 8 in DS goat breeds and 2 in ML goat breed. The most frequentprivate alleles were 246 bp for CSRD247 in ML (0.32) and 177 bp for SRCRSP5 in DS (0.15), respectively.Private alleles of ML and DS goat breeds could be used for traceability of GR dairy products thereforefurther analysis will be performed in order to validate the possible use of these alleles on pool DNA samplesextracted from mono‐breed dairy products.
AB - The establishment of useful analytical methods able to ensure the origin of the products, including thebreed used, are important in maintaining the reliability of these products in order to develop a marketsegment. Traceability, obtained by molecular analysis, could be a reliable proposal for the authenticationand valorization of animal products. In Sicily, the three most important dairy goat breeds are Girgentana(GR), Maltese (ML) and Derivata di Siria (DS). The GR is an endangered autochthonous goat breed.Preservation of endangered breeds could be achieved by establishing economic reasons for their survival.The aim of this work was to verify the use of microsatellite markers to assess a genetic traceability systemto discriminate breeds and to detect adulteration in GR dairy products.A total of 314 individuals belonging to GR, ML, and DS goat breeds were genotyped at 24 microsatellitemarkers and a subset of 9 microsatellites was chosen for further analysis due to the presence of privatealleles. The analyzed markers were FBC48, FBC20, CSRD247, SRCRSP5, OLADRB, SRCRSP8, OARAE54,SRCRSP24, and TGLA122. Fragments analysis of multiplex PCR was performed by capillary electrophoresiswith ABI3130xl Genetic Analyzer. Allele frequency, average number of alleles, allelic richness (AR), Ho andHe, PIC, and HWE for 9 loci were estimated using CERVUS 3.0.3 (Marshall et al., 1998), GENETIX (Belkhir etal., 1996), FSTAT 2.9.3.2 (Goudet, 1995) and POPGENE 1.31 (Yeh et al., 1999) software.The results showed high variability of markers subset considering that mean PIC values was 0.6449 andaverage number of alleles per locus was 8.67; AR ranged from 4.585 in TGLA122 to 8.980 in OLADRB. Sixmicrosatellite markers from subset were not in HWE probably due to heterozygote excess (Ho=0.6133).Intotal 4 private alleles were found in GR, 8 in DS goat breeds and 2 in ML goat breed. The most frequentprivate alleles were 246 bp for CSRD247 in ML (0.32) and 177 bp for SRCRSP5 in DS (0.15), respectively.Private alleles of ML and DS goat breeds could be used for traceability of GR dairy products thereforefurther analysis will be performed in order to validate the possible use of these alleles on pool DNA samplesextracted from mono‐breed dairy products.
UR - http://hdl.handle.net/10447/99744
M3 - Other
SP - 244
EP - 244
ER -