Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method

Vita Di Stefano, Vincenzo Ferrantelli, Nicola Cicero, Andrea Macaluso, Angelina Alongi, Vita Giaccone, Rosa Pitonzo

Risultato della ricerca: Article

Abstract

A simple and efficient method was developed for simultaneousanalysis of five illegal residual β2-agonists in cattle hair. β2-Agonistswere quantified by ultra high performance liquid chromatographycoupled with electrospray ionisation tandem mass spectrometryoperating in positive multiple-reaction monitoring mode. The methodwas validated as quantitative confirmatory method according to theEU Decision 2002/657/EC: instrumental linearity, specificity, precision,recovery, decision limit (CCα) and detection capability (CCβ) wereevaluated. The recovery were greater than 90% and the methodappeared suitable for the control of these β2-agonists in cattle hairsamples with LOQ values between 4.9 and 5.5 μg/kg. This methodcould represent a simple and cheap approach to confirm β2-agonistscontamination of cattle for feeding in a not invasive way and beforeslaughter operations.
Lingua originaleEnglish
Numero di pagine5
RivistaNatural Product Research
Stato di pubblicazionePublished - 2016

Fingerprint

Hair
agonists
hairs
cattle feeding
Recovery
Electrospray ionization
cattle
ionization
quantitative analysis
sampling
liquids
Limit of Detection
Monitoring
monitoring
Liquids
methodology

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

Cita questo

Di Stefano, V., Ferrantelli, V., Cicero, N., Macaluso, A., Alongi, A., Giaccone, V., & Pitonzo, R. (2016). Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method. Natural Product Research.

Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method. / Di Stefano, Vita; Ferrantelli, Vincenzo; Cicero, Nicola; Macaluso, Andrea; Alongi, Angelina; Giaccone, Vita; Pitonzo, Rosa.

In: Natural Product Research, 2016.

Risultato della ricerca: Article

Di Stefano, V, Ferrantelli, V, Cicero, N, Macaluso, A, Alongi, A, Giaccone, V & Pitonzo, R 2016, 'Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method', Natural Product Research.
Di Stefano, Vita ; Ferrantelli, Vincenzo ; Cicero, Nicola ; Macaluso, Andrea ; Alongi, Angelina ; Giaccone, Vita ; Pitonzo, Rosa. / Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method. In: Natural Product Research. 2016.
@article{0ecdcc294cb94c60ad9cd02130ebef11,
title = "Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method",
abstract = "A simple and efficient method was developed for simultaneousanalysis of five illegal residual β2-agonists in cattle hair. β2-Agonistswere quantified by ultra high performance liquid chromatographycoupled with electrospray ionisation tandem mass spectrometryoperating in positive multiple-reaction monitoring mode. The methodwas validated as quantitative confirmatory method according to theEU Decision 2002/657/EC: instrumental linearity, specificity, precision,recovery, decision limit (CCα) and detection capability (CCβ) wereevaluated. The recovery were greater than 90{\%} and the methodappeared suitable for the control of these β2-agonists in cattle hairsamples with LOQ values between 4.9 and 5.5 μg/kg. This methodcould represent a simple and cheap approach to confirm β2-agonistscontamination of cattle for feeding in a not invasive way and beforeslaughter operations.",
author = "{Di Stefano}, Vita and Vincenzo Ferrantelli and Nicola Cicero and Andrea Macaluso and Angelina Alongi and Vita Giaccone and Rosa Pitonzo",
year = "2016",
language = "English",
journal = "Natural Product Research",
issn = "1478-6419",
publisher = "Taylor and Francis Ltd.",

}

TY - JOUR

T1 - Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method

AU - Di Stefano, Vita

AU - Ferrantelli, Vincenzo

AU - Cicero, Nicola

AU - Macaluso, Andrea

AU - Alongi, Angelina

AU - Giaccone, Vita

AU - Pitonzo, Rosa

PY - 2016

Y1 - 2016

N2 - A simple and efficient method was developed for simultaneousanalysis of five illegal residual β2-agonists in cattle hair. β2-Agonistswere quantified by ultra high performance liquid chromatographycoupled with electrospray ionisation tandem mass spectrometryoperating in positive multiple-reaction monitoring mode. The methodwas validated as quantitative confirmatory method according to theEU Decision 2002/657/EC: instrumental linearity, specificity, precision,recovery, decision limit (CCα) and detection capability (CCβ) wereevaluated. The recovery were greater than 90% and the methodappeared suitable for the control of these β2-agonists in cattle hairsamples with LOQ values between 4.9 and 5.5 μg/kg. This methodcould represent a simple and cheap approach to confirm β2-agonistscontamination of cattle for feeding in a not invasive way and beforeslaughter operations.

AB - A simple and efficient method was developed for simultaneousanalysis of five illegal residual β2-agonists in cattle hair. β2-Agonistswere quantified by ultra high performance liquid chromatographycoupled with electrospray ionisation tandem mass spectrometryoperating in positive multiple-reaction monitoring mode. The methodwas validated as quantitative confirmatory method according to theEU Decision 2002/657/EC: instrumental linearity, specificity, precision,recovery, decision limit (CCα) and detection capability (CCβ) wereevaluated. The recovery were greater than 90% and the methodappeared suitable for the control of these β2-agonists in cattle hairsamples with LOQ values between 4.9 and 5.5 μg/kg. This methodcould represent a simple and cheap approach to confirm β2-agonistscontamination of cattle for feeding in a not invasive way and beforeslaughter operations.

UR - http://hdl.handle.net/10447/213269

M3 - Article

JO - Natural Product Research

JF - Natural Product Research

SN - 1478-6419

ER -