An old method for good new cells

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The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).
Lingua originaleEnglish
pagine (da-a)1116-1118
Numero di pagine3
RivistaTransplantation Proceedings
Stato di pubblicazionePublished - 2009

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

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