An integrated technological approach to the selection of lactic acid bacteria of flour origin for sourdough production

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Several lactic acid bacteria (LAB) were evaluated in situ for their potential in sourdough fermentation. Thestrains belonged to Lactobacillus plantarum, Lactobacillus sakei, Lactobacillus sanfranciscensis, Leuconostoc citreum,Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides and Weissella cibaria. LAB were used, in individualinocula, to carry out the fermentation of γ-ray treated (sterile) flour and untreated commercial flour, in orderto evaluate their performances both in the absence and presence of the native microbiota of flour. The pH andtotal titratable acidity (TTA) showed a strong and fast acidification of the experimental sourdough determinedby W. cibaria and Ln. citreum strains. All strains were followed during fermentation by plate count. Randomlyamplified polymorphic DNA (RAPD)-PCR analysis applied on the colonies isolated from the highest dilution ofsamples confirmed the dominance of the added strains in all sourdoughs prepared with sterile and non-sterileflour. The analysis of organic acids, performed by high-performance liquid chromatography (HPLC), confirmedthat some W. cibaria and Ln. citreum strains showed an optimal fermentation quotient. The volatile organiccompound (VOC) composition resulting from the gas chromatography coupled with mass spectrometry (GC/MS)analysis of sourdough headspace recognised 51 chemical compounds including acids, alcohols, aldehydes, esters,ketones, lactones, acetate, alkane, and phenol, most of which are of LAB origin and are relevant for the final bread.After baking, the breads were evaluated for the height of the central slices, colour of crust and crumb, hardnessand number and distribution of alveolus. The combination of these results indicated that strains Ln. citreumPON10079 and PON10080 and W. cibaria PON10030 and PON10032 are suitable cultures to use in industrialproduction.
Lingua originaleEnglish
pagine (da-a)1569-1578
Numero di pagine10
RivistaFood Research International
Stato di pubblicazionePublished - 2013

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