Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer

Flora Iovino, Giorgio Stassi, Benjamin Esterni, Mark Diebel, Yi Xiao, Jocelyne Jacquemier, Patrice Viens, Mark Diebel, Gabriela Dontu, Carole Tarpin, Christophe Ginestier, Emmanuelle Charafe-Jauffret, Giorgio Stassi, François Bertucci, Jean-Marc Extra, Sanford H. Barsky, Max S. Wicha, Luc Xerri, Gilles Houvenaeghel, Daniel BirnbaumFlora Iovino

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545 Citazioni (Scopus)


Purpose: To examine the role of cancer stem cells (CSC) in mediating metastasis in inflammatory breast cancer (IBC) and the association of these cells with patient outcome in this aggressive type of breast cancer. Experimental Design: CSCs were isolated from SUM149 and MARY-X, an IBC cell line and primary xenograft, by virtue of increased aldehyde dehydrogenase (ALDH) activity as assessed by the ALDEFLUOR assay. Invasion and metastasis of CSC populations were assessed by in vitro and mouse xenograft assays. Expression of ALDH1 was determined on a retrospective series of 109 IBC patients and this was correlated with histoclinical data. All statistical tests were two sided. Log-rank tests using Kaplan-Meier analysis were used to determine the correlation of ALDH1 expression with development of metastasis and patient outcome. Results: Both in vitro and xenograft assays showed that invasion and metastasis in IBC are mediated by a cellular component that displays ALDH activity. Furthermore, expression of ALDH1 in IBC was an independent predictive factor for early metastasis and decreased survival in this patient population. Conclusions: These results suggest that the metastatic, aggressive behavior of IBC may be mediated by a CSC component that displays ALDH enzymatic activity. ALDH1 expression represents the first independent prognostic marker to predict metastasis and poor patient outcome in IBC. The results illustrate how stem cell research can translate into clinical practice in the IBC field.
Lingua originaleEnglish
pagine (da-a)45-55
Numero di pagine11
RivistaClinical Cancer Research
Stato di pubblicazionePublished - 2010

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1300.1306???


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