ALCOHOLIC LIVER DISEASE: A MOUSE MODEL REVEALS PROTECTION BY LACTOBACILLUS FERMENTUM

Antonella Marino Gammazza, Claudia Sangiorgi, Valentina Di Felice, Celeste Caruso Bavisotto, Francesco Cappello, Rosario Barone, Francesca Rappa, Felicia Farina, Giovanni Zummo, Giovanni Tomasello, Antonella Marino Gammazza, Filippo Macaluso, Rosario Barone, Massimo Cocchi, Francesca Rappa, Giovanni Tomasello, Celeste Caruso Bavisotto, Everly Conway De Macario, Francesco Cappello, Alberto J.L. MacarioValentina Di Felice

Risultato della ricerca: Article

20 Citazioni (Scopus)

Abstract

Objectives: Alcoholism is one of the most devasting diseases with high incidence, but knowledge of its pathology and treatment is still plagued with gaps mostly because of the inherent limitations of research with patients. we developed an animal model for studying liver histopathology, Hsp /heat-shock protein)-chaperones involvemnent, and response to treatment. Methods: The system was standardized using mice to which ethanol was orally administered alone or in combination with lactobacillus fermentum following a precise schedule over time and applying, at predetermined intervals, a battery of techniques (histology, immunohistochemistry, western blotting, real-time PCR, immunoprecipitation, 3-nitrosyne labelling) to assess liver pathology (e.g., steatosius, fibrosis), and Hsp60 and iNOS (inducible form of nitric oxide synthase) gene expression and protein levels, and post-translational modifications. Results: Typical ethanol-induced liuver pathology occurred and the effect of the probiotic coulkd be reliably monitored. steatosis score, iNOS levels, and nitrate proteins (e.g. Hsp60) decreased after probiotic intake. Conclusions: We describe a mouse model useful for studying liver disease induced by chronic ethanol intake and for testing pertinent therapeutic agents, e.g., probiotics. we tested L. fermentum which reduced considerably ethanol-induced tissue damage and deleterious post-translational modifications of the chaperone Hsp60. The model is available to test other agents and probiotics with therapeutic potential in alcoholic liver disease.
Lingua originaleEnglish
Numero di pagine13
RivistaDefault journal
Stato di pubblicazionePublished - 2016

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Lactobacillus fermentum
Alcoholic Liver Diseases
Probiotics
Ethanol
Nitric Oxide Synthase Type II
Post Translational Protein Processing
Pathology
Chaperonin 60
Liver
Therapeutics
Heat-Shock Proteins
Immunoprecipitation
Nitrates
Alcoholism
Liver Diseases
Real-Time Polymerase Chain Reaction
Histology
Appointments and Schedules
Fibrosis
Animal Models

All Science Journal Classification (ASJC) codes

  • Gastroenterology

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ALCOHOLIC LIVER DISEASE: A MOUSE MODEL REVEALS PROTECTION BY LACTOBACILLUS FERMENTUM. / Marino Gammazza, Antonella; Sangiorgi, Claudia; Di Felice, Valentina; Caruso Bavisotto, Celeste; Cappello, Francesco; Barone, Rosario; Rappa, Francesca; Farina, Felicia; Zummo, Giovanni; Tomasello, Giovanni; Gammazza, Antonella Marino; Macaluso, Filippo; Barone, Rosario; Cocchi, Massimo; Rappa, Francesca; Tomasello, Giovanni; Bavisotto, Celeste Caruso; De Macario, Everly Conway; Cappello, Francesco; Macario, Alberto J.L.; Di Felice, Valentina.

In: Default journal, 2016.

Risultato della ricerca: Article

Marino Gammazza, A, Sangiorgi, C, Di Felice, V, Caruso Bavisotto, C, Cappello, F, Barone, R, Rappa, F, Farina, F, Zummo, G, Tomasello, G, Gammazza, AM, Macaluso, F, Barone, R, Cocchi, M, Rappa, F, Tomasello, G, Bavisotto, CC, De Macario, EC, Cappello, F, Macario, AJL & Di Felice, V 2016, 'ALCOHOLIC LIVER DISEASE: A MOUSE MODEL REVEALS PROTECTION BY LACTOBACILLUS FERMENTUM', Default journal.
Marino Gammazza, Antonella ; Sangiorgi, Claudia ; Di Felice, Valentina ; Caruso Bavisotto, Celeste ; Cappello, Francesco ; Barone, Rosario ; Rappa, Francesca ; Farina, Felicia ; Zummo, Giovanni ; Tomasello, Giovanni ; Gammazza, Antonella Marino ; Macaluso, Filippo ; Barone, Rosario ; Cocchi, Massimo ; Rappa, Francesca ; Tomasello, Giovanni ; Bavisotto, Celeste Caruso ; De Macario, Everly Conway ; Cappello, Francesco ; Macario, Alberto J.L. ; Di Felice, Valentina. / ALCOHOLIC LIVER DISEASE: A MOUSE MODEL REVEALS PROTECTION BY LACTOBACILLUS FERMENTUM. In: Default journal. 2016.
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title = "ALCOHOLIC LIVER DISEASE: A MOUSE MODEL REVEALS PROTECTION BY LACTOBACILLUS FERMENTUM",
abstract = "Objectives: Alcoholism is one of the most devasting diseases with high incidence, but knowledge of its pathology and treatment is still plagued with gaps mostly because of the inherent limitations of research with patients. we developed an animal model for studying liver histopathology, Hsp /heat-shock protein)-chaperones involvemnent, and response to treatment. Methods: The system was standardized using mice to which ethanol was orally administered alone or in combination with lactobacillus fermentum following a precise schedule over time and applying, at predetermined intervals, a battery of techniques (histology, immunohistochemistry, western blotting, real-time PCR, immunoprecipitation, 3-nitrosyne labelling) to assess liver pathology (e.g., steatosius, fibrosis), and Hsp60 and iNOS (inducible form of nitric oxide synthase) gene expression and protein levels, and post-translational modifications. Results: Typical ethanol-induced liuver pathology occurred and the effect of the probiotic coulkd be reliably monitored. steatosis score, iNOS levels, and nitrate proteins (e.g. Hsp60) decreased after probiotic intake. Conclusions: We describe a mouse model useful for studying liver disease induced by chronic ethanol intake and for testing pertinent therapeutic agents, e.g., probiotics. we tested L. fermentum which reduced considerably ethanol-induced tissue damage and deleterious post-translational modifications of the chaperone Hsp60. The model is available to test other agents and probiotics with therapeutic potential in alcoholic liver disease.",
author = "{Marino Gammazza}, Antonella and Claudia Sangiorgi and {Di Felice}, Valentina and {Caruso Bavisotto}, Celeste and Francesco Cappello and Rosario Barone and Francesca Rappa and Felicia Farina and Giovanni Zummo and Giovanni Tomasello and Gammazza, {Antonella Marino} and Filippo Macaluso and Rosario Barone and Massimo Cocchi and Francesca Rappa and Giovanni Tomasello and Bavisotto, {Celeste Caruso} and {De Macario}, {Everly Conway} and Francesco Cappello and Macario, {Alberto J.L.} and {Di Felice}, Valentina",
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T1 - ALCOHOLIC LIVER DISEASE: A MOUSE MODEL REVEALS PROTECTION BY LACTOBACILLUS FERMENTUM

AU - Marino Gammazza, Antonella

AU - Sangiorgi, Claudia

AU - Di Felice, Valentina

AU - Caruso Bavisotto, Celeste

AU - Cappello, Francesco

AU - Barone, Rosario

AU - Rappa, Francesca

AU - Farina, Felicia

AU - Zummo, Giovanni

AU - Tomasello, Giovanni

AU - Gammazza, Antonella Marino

AU - Macaluso, Filippo

AU - Barone, Rosario

AU - Cocchi, Massimo

AU - Rappa, Francesca

AU - Tomasello, Giovanni

AU - Bavisotto, Celeste Caruso

AU - De Macario, Everly Conway

AU - Cappello, Francesco

AU - Macario, Alberto J.L.

AU - Di Felice, Valentina

PY - 2016

Y1 - 2016

N2 - Objectives: Alcoholism is one of the most devasting diseases with high incidence, but knowledge of its pathology and treatment is still plagued with gaps mostly because of the inherent limitations of research with patients. we developed an animal model for studying liver histopathology, Hsp /heat-shock protein)-chaperones involvemnent, and response to treatment. Methods: The system was standardized using mice to which ethanol was orally administered alone or in combination with lactobacillus fermentum following a precise schedule over time and applying, at predetermined intervals, a battery of techniques (histology, immunohistochemistry, western blotting, real-time PCR, immunoprecipitation, 3-nitrosyne labelling) to assess liver pathology (e.g., steatosius, fibrosis), and Hsp60 and iNOS (inducible form of nitric oxide synthase) gene expression and protein levels, and post-translational modifications. Results: Typical ethanol-induced liuver pathology occurred and the effect of the probiotic coulkd be reliably monitored. steatosis score, iNOS levels, and nitrate proteins (e.g. Hsp60) decreased after probiotic intake. Conclusions: We describe a mouse model useful for studying liver disease induced by chronic ethanol intake and for testing pertinent therapeutic agents, e.g., probiotics. we tested L. fermentum which reduced considerably ethanol-induced tissue damage and deleterious post-translational modifications of the chaperone Hsp60. The model is available to test other agents and probiotics with therapeutic potential in alcoholic liver disease.

AB - Objectives: Alcoholism is one of the most devasting diseases with high incidence, but knowledge of its pathology and treatment is still plagued with gaps mostly because of the inherent limitations of research with patients. we developed an animal model for studying liver histopathology, Hsp /heat-shock protein)-chaperones involvemnent, and response to treatment. Methods: The system was standardized using mice to which ethanol was orally administered alone or in combination with lactobacillus fermentum following a precise schedule over time and applying, at predetermined intervals, a battery of techniques (histology, immunohistochemistry, western blotting, real-time PCR, immunoprecipitation, 3-nitrosyne labelling) to assess liver pathology (e.g., steatosius, fibrosis), and Hsp60 and iNOS (inducible form of nitric oxide synthase) gene expression and protein levels, and post-translational modifications. Results: Typical ethanol-induced liuver pathology occurred and the effect of the probiotic coulkd be reliably monitored. steatosis score, iNOS levels, and nitrate proteins (e.g. Hsp60) decreased after probiotic intake. Conclusions: We describe a mouse model useful for studying liver disease induced by chronic ethanol intake and for testing pertinent therapeutic agents, e.g., probiotics. we tested L. fermentum which reduced considerably ethanol-induced tissue damage and deleterious post-translational modifications of the chaperone Hsp60. The model is available to test other agents and probiotics with therapeutic potential in alcoholic liver disease.

UR - http://hdl.handle.net/10447/168159

M3 - Article

JO - Default journal

JF - Default journal

ER -