Osteoblastic-like cells (SAOS-2) wereseeded on three differently treated surfaces of Ti-6Al-4V (T1: not treated; T2: chemically treated;T3: electrochemically treated). After 6h, the cellsgrown on T1 and T2 surfaces showed a typicalpoligonal morphology, they were stronglyadherent to the surface and after 24h-48h theyspread and formed a confluent monolayer. On thecontrary, cells seeded on T3 showedheterogeneous morphology, decreased adhesion,loss of thin cellular expansions, and showedcellular shrinkage and apoptotic blebbing; onlyafter 48h they were able to attach and form amonolayer after 4 days of seeding. Cellproliferation was significantly lower on T3 thanT1 and T2 surfaces. After 4 days of culture, thecells grown on T3 surface, recovered as adhesionefficiency and proliferation rate showing a betteradaptation to the surface. Western Blottinganalysis didn’t reveal any HSP70 proteininduction in all three surfaces. Furtherinvestigations will better analyze if this reducedearly adhesion could be related to themaintainance of a cell differentiation and/or to analtered production of adhesion molecules.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2004|