Activation of angiotensin II type 1 receptors and contractile activity in human sigmoid colon in vitro

Flavia Mule', Michelangelo Auteri, Rosa Maria Serio, Maria Grazia Zizzo, Maria Grazia Zizzo, Mastropaolo, Auteri, Liotta, Flavia Mulè, Rosa Serio, Caldara

Risultato della ricerca: Article

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Abstract

Aim: To analyse the effects of angiotensin II (Ang II) on the contractility of human sigmoid colon, and to characterize the subtype(s) of receptor(s) involved and the related action mechanism. Methods: The contractility of sigmoid colon circular muscle strips was recorded isometrically. RT-PCR and immunohistochemistry were used to reveal the eventual existence of a local renin-angiotensin system (RAS) and the distribution of Ang II receptors. Results: Transcripts encoding for the Ang II type 1 (AT<inf>1</inf>) and the Ang II type 2 (AT<inf>2</inf>) receptor subtypes and for the angiotensin-converting enzyme in the whole-thickness muscular wall were observed. Ang II caused a concentration-dependent contractile response, which is antagonized by losartan, AT<inf>1</inf> receptor antagonist, but not by PD123319, AT<inf>2</inf> receptor antagonist. The joint application of losartan and PD123319 did not produce any additive effect. The contractile response to Ang II was partially reduced by tetrodotoxin, Na<sup>+</sup> voltage-gated neural channel blocker, and to some extent by SR48968, tachykinin NK<inf>2</inf> receptor antagonist. However, hexamethonium, nicotinic receptor antagonist, atropine, cholinergic muscarinic receptor antagonist and SR140333, tachykinin NK<inf>1</inf> receptor antagonist, were ineffective. Immunohistochemical analysis showed that AT<inf>1</inf> receptors were expressed on the smooth muscle layers and myenteric plexus. Conclusion: Ang II positively modulates the spontaneous contractile activity of human sigmoid colon via activation of post-junctional and pre-junctional AT<inf>1</inf> receptors, the latter located on the enteric nerves that modulate the release of tachykinins. The presence of the components of RAS in the human colon suggests that Ang II can be also locally generated to control colonic motility.
Lingua originaleEnglish
pagine (da-a)37-45
Numero di pagine9
RivistaActa Physiologica
Volume215
Stato di pubblicazionePublished - 2015

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Angiotensin Type 1 Receptor
Sigmoid Colon
Human Activities
Angiotensin II
Tachykinin Receptors
Losartan
Renin-Angiotensin System
Angiotensin Type 2 Receptor
Nicotinic Antagonists
Tachykinins
Myenteric Plexus
Hexamethonium
Angiotensin Receptors
Muscarinic Antagonists
Tetrodotoxin
Nicotinic Receptors
Cholinergic Antagonists
Muscarinic Receptors
Peptidyl-Dipeptidase A
Atropine

All Science Journal Classification (ASJC) codes

  • Physiology

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Activation of angiotensin II type 1 receptors and contractile activity in human sigmoid colon in vitro. / Mule', Flavia; Auteri, Michelangelo; Serio, Rosa Maria; Zizzo, Maria Grazia; Zizzo, Maria Grazia; Mastropaolo; Auteri; Liotta; Mulè, Flavia; Serio, Rosa; Caldara.

In: Acta Physiologica, Vol. 215, 2015, pag. 37-45.

Risultato della ricerca: Article

Mule', Flavia ; Auteri, Michelangelo ; Serio, Rosa Maria ; Zizzo, Maria Grazia ; Zizzo, Maria Grazia ; Mastropaolo ; Auteri ; Liotta ; Mulè, Flavia ; Serio, Rosa ; Caldara. / Activation of angiotensin II type 1 receptors and contractile activity in human sigmoid colon in vitro. In: Acta Physiologica. 2015 ; Vol. 215. pagg. 37-45.
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abstract = "Aim: To analyse the effects of angiotensin II (Ang II) on the contractility of human sigmoid colon, and to characterize the subtype(s) of receptor(s) involved and the related action mechanism. Methods: The contractility of sigmoid colon circular muscle strips was recorded isometrically. RT-PCR and immunohistochemistry were used to reveal the eventual existence of a local renin-angiotensin system (RAS) and the distribution of Ang II receptors. Results: Transcripts encoding for the Ang II type 1 (AT1) and the Ang II type 2 (AT2) receptor subtypes and for the angiotensin-converting enzyme in the whole-thickness muscular wall were observed. Ang II caused a concentration-dependent contractile response, which is antagonized by losartan, AT1 receptor antagonist, but not by PD123319, AT2 receptor antagonist. The joint application of losartan and PD123319 did not produce any additive effect. The contractile response to Ang II was partially reduced by tetrodotoxin, Na+ voltage-gated neural channel blocker, and to some extent by SR48968, tachykinin NK2 receptor antagonist. However, hexamethonium, nicotinic receptor antagonist, atropine, cholinergic muscarinic receptor antagonist and SR140333, tachykinin NK1 receptor antagonist, were ineffective. Immunohistochemical analysis showed that AT1 receptors were expressed on the smooth muscle layers and myenteric plexus. Conclusion: Ang II positively modulates the spontaneous contractile activity of human sigmoid colon via activation of post-junctional and pre-junctional AT1 receptors, the latter located on the enteric nerves that modulate the release of tachykinins. The presence of the components of RAS in the human colon suggests that Ang II can be also locally generated to control colonic motility.",
author = "Flavia Mule' and Michelangelo Auteri and Serio, {Rosa Maria} and Zizzo, {Maria Grazia} and Zizzo, {Maria Grazia} and Mastropaolo and Auteri and Liotta and Flavia Mul{\`e} and Rosa Serio and Caldara",
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T1 - Activation of angiotensin II type 1 receptors and contractile activity in human sigmoid colon in vitro

AU - Mule', Flavia

AU - Auteri, Michelangelo

AU - Serio, Rosa Maria

AU - Zizzo, Maria Grazia

AU - Zizzo, Maria Grazia

AU - Mastropaolo, null

AU - Auteri, null

AU - Liotta, null

AU - Mulè, Flavia

AU - Serio, Rosa

AU - Caldara, null

PY - 2015

Y1 - 2015

N2 - Aim: To analyse the effects of angiotensin II (Ang II) on the contractility of human sigmoid colon, and to characterize the subtype(s) of receptor(s) involved and the related action mechanism. Methods: The contractility of sigmoid colon circular muscle strips was recorded isometrically. RT-PCR and immunohistochemistry were used to reveal the eventual existence of a local renin-angiotensin system (RAS) and the distribution of Ang II receptors. Results: Transcripts encoding for the Ang II type 1 (AT1) and the Ang II type 2 (AT2) receptor subtypes and for the angiotensin-converting enzyme in the whole-thickness muscular wall were observed. Ang II caused a concentration-dependent contractile response, which is antagonized by losartan, AT1 receptor antagonist, but not by PD123319, AT2 receptor antagonist. The joint application of losartan and PD123319 did not produce any additive effect. The contractile response to Ang II was partially reduced by tetrodotoxin, Na+ voltage-gated neural channel blocker, and to some extent by SR48968, tachykinin NK2 receptor antagonist. However, hexamethonium, nicotinic receptor antagonist, atropine, cholinergic muscarinic receptor antagonist and SR140333, tachykinin NK1 receptor antagonist, were ineffective. Immunohistochemical analysis showed that AT1 receptors were expressed on the smooth muscle layers and myenteric plexus. Conclusion: Ang II positively modulates the spontaneous contractile activity of human sigmoid colon via activation of post-junctional and pre-junctional AT1 receptors, the latter located on the enteric nerves that modulate the release of tachykinins. The presence of the components of RAS in the human colon suggests that Ang II can be also locally generated to control colonic motility.

AB - Aim: To analyse the effects of angiotensin II (Ang II) on the contractility of human sigmoid colon, and to characterize the subtype(s) of receptor(s) involved and the related action mechanism. Methods: The contractility of sigmoid colon circular muscle strips was recorded isometrically. RT-PCR and immunohistochemistry were used to reveal the eventual existence of a local renin-angiotensin system (RAS) and the distribution of Ang II receptors. Results: Transcripts encoding for the Ang II type 1 (AT1) and the Ang II type 2 (AT2) receptor subtypes and for the angiotensin-converting enzyme in the whole-thickness muscular wall were observed. Ang II caused a concentration-dependent contractile response, which is antagonized by losartan, AT1 receptor antagonist, but not by PD123319, AT2 receptor antagonist. The joint application of losartan and PD123319 did not produce any additive effect. The contractile response to Ang II was partially reduced by tetrodotoxin, Na+ voltage-gated neural channel blocker, and to some extent by SR48968, tachykinin NK2 receptor antagonist. However, hexamethonium, nicotinic receptor antagonist, atropine, cholinergic muscarinic receptor antagonist and SR140333, tachykinin NK1 receptor antagonist, were ineffective. Immunohistochemical analysis showed that AT1 receptors were expressed on the smooth muscle layers and myenteric plexus. Conclusion: Ang II positively modulates the spontaneous contractile activity of human sigmoid colon via activation of post-junctional and pre-junctional AT1 receptors, the latter located on the enteric nerves that modulate the release of tachykinins. The presence of the components of RAS in the human colon suggests that Ang II can be also locally generated to control colonic motility.

UR - http://hdl.handle.net/10447/155064

UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1748-1716

M3 - Article

VL - 215

SP - 37

EP - 45

JO - Acta Physiologica Scandinavica

JF - Acta Physiologica Scandinavica

SN - 0370-839X

ER -