Abnormal mitotic spindle assembly and cytokinesis induced by D-Limonene in cultured mammalian cells

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Abstract

d-Limonene is found widely in citrus and many other plant species; it is a major constituent of many essential oils and is used as a solvent for commercial purposes. With the discovery of its chemotherapeutic properties against cancer, it is important to investigate the biological effects of the exposure to d-Limonene and elucidate its, as yet unknown, mechanism of action. We reported here that d-Limonene is toxic in V79 Chinese hamster cells in a dose-dependent manner. Moreover, to determine the cellular target of d-Limonene, we performed morphological observations and immunocytochemical analysis and we showed that this drug has a direct effect on dividing cells preventing assembly of mitotic spindle microtubules. This affects both chromosome segregation and cytokinesis, resulting in aneuploidy that in turn can lead to cell death or genomic instability.
Lingua originaleEnglish
pagine (da-a)1-5
Numero di pagine5
RivistaDefault journal
VolumeLuglio 2013
Stato di pubblicazionePublished - 2013

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Spindle Apparatus
Cytokinesis
Cultured Cells
Cells
Chromosome Segregation
Citrus
Genomic Instability
Poisons
Aneuploidy
Cell death
Volatile Oils
Chromosomes
Cricetulus
Microtubules
Cell Death
limonene
Pharmaceutical Preparations
Neoplasms

All Science Journal Classification (ASJC) codes

  • Genetics
  • Toxicology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

Cita questo

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title = "Abnormal mitotic spindle assembly and cytokinesis induced by D-Limonene in cultured mammalian cells",
abstract = "d-Limonene is found widely in citrus and many other plant species; it is a major constituent of many essential oils and is used as a solvent for commercial purposes. With the discovery of its chemotherapeutic properties against cancer, it is important to investigate the biological effects of the exposure to d-Limonene and elucidate its, as yet unknown, mechanism of action. We reported here that d-Limonene is toxic in V79 Chinese hamster cells in a dose-dependent manner. Moreover, to determine the cellular target of d-Limonene, we performed morphological observations and immunocytochemical analysis and we showed that this drug has a direct effect on dividing cells preventing assembly of mitotic spindle microtubules. This affects both chromosome segregation and cytokinesis, resulting in aneuploidy that in turn can lead to cell death or genomic instability.",
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T1 - Abnormal mitotic spindle assembly and cytokinesis induced by D-Limonene in cultured mammalian cells

AU - Caradonna, Fabio

AU - Sciandrello, Giulia

AU - Catanzaro, Irene

AU - Naselli, Flores

AU - Mauro, Maurizio

PY - 2013

Y1 - 2013

N2 - d-Limonene is found widely in citrus and many other plant species; it is a major constituent of many essential oils and is used as a solvent for commercial purposes. With the discovery of its chemotherapeutic properties against cancer, it is important to investigate the biological effects of the exposure to d-Limonene and elucidate its, as yet unknown, mechanism of action. We reported here that d-Limonene is toxic in V79 Chinese hamster cells in a dose-dependent manner. Moreover, to determine the cellular target of d-Limonene, we performed morphological observations and immunocytochemical analysis and we showed that this drug has a direct effect on dividing cells preventing assembly of mitotic spindle microtubules. This affects both chromosome segregation and cytokinesis, resulting in aneuploidy that in turn can lead to cell death or genomic instability.

AB - d-Limonene is found widely in citrus and many other plant species; it is a major constituent of many essential oils and is used as a solvent for commercial purposes. With the discovery of its chemotherapeutic properties against cancer, it is important to investigate the biological effects of the exposure to d-Limonene and elucidate its, as yet unknown, mechanism of action. We reported here that d-Limonene is toxic in V79 Chinese hamster cells in a dose-dependent manner. Moreover, to determine the cellular target of d-Limonene, we performed morphological observations and immunocytochemical analysis and we showed that this drug has a direct effect on dividing cells preventing assembly of mitotic spindle microtubules. This affects both chromosome segregation and cytokinesis, resulting in aneuploidy that in turn can lead to cell death or genomic instability.

UR - http://hdl.handle.net/10447/84330

UR - http://www.ncbi.nlm.nih.gov/pubmed/23913329

M3 - Article

VL - Luglio 2013

SP - 1

EP - 5

JO - Default journal

JF - Default journal

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