Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 159- |
| Numero di pagine | 11 |
| Rivista | Marine Drugs |
| Volume | 17 |
| Stato di pubblicazione | Published - 2019 |
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All Science Journal Classification (ASJC) codes
- Drug Discovery
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A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes. / Arizza, Vincenzo; Schillaci, Domenico; Cusimano, Maria Grazia; Barone, Giampaolo; Spinello, Angelo; Cascioferro, Stella Maria; Parrino, Barbara; Vitale, Maria; Spinello, Angelo; Magistrato, Alessandra.
In: Marine Drugs, Vol. 17, 2019, pag. 159-.Risultato della ricerca: Article
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TY - JOUR
T1 - A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes
AU - Arizza, Vincenzo
AU - Schillaci, Domenico
AU - Cusimano, Maria Grazia
AU - Barone, Giampaolo
AU - Spinello, Angelo
AU - Cascioferro, Stella Maria
AU - Parrino, Barbara
AU - Vitale, Maria
AU - Spinello, Angelo
AU - Magistrato, Alessandra
PY - 2019
Y1 - 2019
N2 - Due to the limited number of available antibiotics, antimicrobial peptides (AMPs) are considered antimicrobial candidates to fight difficult-to-treat infections such as those associated with biofilms. Marine environments are precious sources of AMPs, as shown by the recent discovery of antibiofilm properties of Holothuroidin 2 (H2), an AMP produced by the Mediterranean sea cucumber Holothuria tubulosa. In this study, we considered the properties of a new H2 derivative, named H2d, and we tested it against seven strains of the dangerous foodborne pathogen Listeria monocytogenes. This peptide was more active than H2 in inhibiting the growth of planktonic L. monocytogenes and was able to interfere with biofilm formation at sub-minimum inhibitory concentrations (MICs). Atomic-level molecular dynamics (MD) simulations revealed insights related to the enhanced inhibitory activity of H2d, showing that the peptide is characterized by a more defined tertiary structure with respect to its ancestor. This allows the peptide to better exhibit an amphipathic character, which is an essential requirement for the interaction with cell membranes, similarly to other AMPs. Altogether, these results support the potential use of our synthetic peptide, H2d, as a template for the development of novel AMP-based drugs able to fight foodborne that are resistant to conventional antibiotics.
AB - Due to the limited number of available antibiotics, antimicrobial peptides (AMPs) are considered antimicrobial candidates to fight difficult-to-treat infections such as those associated with biofilms. Marine environments are precious sources of AMPs, as shown by the recent discovery of antibiofilm properties of Holothuroidin 2 (H2), an AMP produced by the Mediterranean sea cucumber Holothuria tubulosa. In this study, we considered the properties of a new H2 derivative, named H2d, and we tested it against seven strains of the dangerous foodborne pathogen Listeria monocytogenes. This peptide was more active than H2 in inhibiting the growth of planktonic L. monocytogenes and was able to interfere with biofilm formation at sub-minimum inhibitory concentrations (MICs). Atomic-level molecular dynamics (MD) simulations revealed insights related to the enhanced inhibitory activity of H2d, showing that the peptide is characterized by a more defined tertiary structure with respect to its ancestor. This allows the peptide to better exhibit an amphipathic character, which is an essential requirement for the interaction with cell membranes, similarly to other AMPs. Altogether, these results support the potential use of our synthetic peptide, H2d, as a template for the development of novel AMP-based drugs able to fight foodborne that are resistant to conventional antibiotics.
UR - http://hdl.handle.net/10447/349767
M3 - Article
VL - 17
SP - 159-
JO - Marine Drugs
JF - Marine Drugs
SN - 1660-3397
ER -