A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data

Gene expression microarrays allow a researcher to measure the simultaneousresponse of thousands of genes to external conditions. Affymetrix GeneChipr expressionarray technology has become a standard tool in medical research. Anyway, a preprocessingstep is usually necessary in order to obtain a gene expression measure. Aim of thispaper is to propose a calibration method to estimate the nominal concentration based on anon-linear mixed model. This method is an enhancement of a method proposed in Mineoet al. (2006). The relationship between raw intensities and concentration is obtained byusing the Langmuir isotherm theory.