Gene expression microarrays allow a researcher to measure the simultaneousresponse of thousands of genes to external conditions. Affymetrix GeneChipr expressionarray technology has become a standard tool in medical research. Anyway, a preprocessingstep is usually necessary in order to obtain a gene expression measure. Aim of thispaper is to propose a calibration method to estimate the nominal concentration based on anon-linear mixed model. This method is an enhancement of a method proposed in Mineoet al. (2006). The relationship between raw intensities and concentration is obtained byusing the Langmuir isotherm theory.
title = "A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data",
abstract = "Gene expression microarrays allow a researcher to measure the simultaneousresponse of thousands of genes to external conditions. Affymetrix GeneChipr expressionarray technology has become a standard tool in medical research. Anyway, a preprocessingstep is usually necessary in order to obtain a gene expression measure. Aim of thispaper is to propose a calibration method to estimate the nominal concentration based on anon-linear mixed model. This method is an enhancement of a method proposed in Mineoet al. (2006). The relationship between raw intensities and concentration is obtained byusing the Langmuir isotherm theory.",
author = "Mariantonietta Ruggieri and Angelo Mineo and Luigi Augugliaro",
year = "2007",
language = "English",
pages = "97--100",
}
TY - CONF
T1 - A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data
AU - Ruggieri, Mariantonietta
AU - Mineo, Angelo
AU - Augugliaro, Luigi
PY - 2007
Y1 - 2007
N2 - Gene expression microarrays allow a researcher to measure the simultaneousresponse of thousands of genes to external conditions. Affymetrix GeneChipr expressionarray technology has become a standard tool in medical research. Anyway, a preprocessingstep is usually necessary in order to obtain a gene expression measure. Aim of thispaper is to propose a calibration method to estimate the nominal concentration based on anon-linear mixed model. This method is an enhancement of a method proposed in Mineoet al. (2006). The relationship between raw intensities and concentration is obtained byusing the Langmuir isotherm theory.
AB - Gene expression microarrays allow a researcher to measure the simultaneousresponse of thousands of genes to external conditions. Affymetrix GeneChipr expressionarray technology has become a standard tool in medical research. Anyway, a preprocessingstep is usually necessary in order to obtain a gene expression measure. Aim of thispaper is to propose a calibration method to estimate the nominal concentration based on anon-linear mixed model. This method is an enhancement of a method proposed in Mineoet al. (2006). The relationship between raw intensities and concentration is obtained byusing the Langmuir isotherm theory.