We applied digital spatial profiling for 87 immune and stromal genes to lymphnode germinal center (GC) dark- and light-zone (DZ/LZ) regions of interest toobtain a differential signature of these two distinct microenvironments. Thespatially resolved 53-genes signature, comprising key genes of the DZ mutationalmachinery and LZ immune and mesenchymal milieu, was applied to the transcriptomes of 543 GC-related diffuse large B cell lymphomas and double-hit (DH) lymphomas. According to the DZ/LZ signature, the GC-related lymphomas were subclassified into two clusters. The subgroups differed in the distribution of DH casesand survival, with most DH displaying a distinct DZ-like profile. The clusteringanalysis was also performed using a 25-genes signature composed of genes positively enriched in the non-B, stromal sub-compartments, for the first timeachieving DZ/LZ discrimination based on stromal/immune features. The reportoffers new insight into the GC microenvironment, hinting at a DZ microenvironment of origin in DH lymphomas.
|Numero di pagine||18|
|Stato di pubblicazione||Published - 2020|
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