A soluble biocompatible guanidine-containing polyamidoamine as promoter of primary brain cell adhesion andin vitrocell culturing

Nicolò Mauro, Fabio Bianco, Nicolò Mauro, Noemi Tonna, Cinzia Cagnoli, Elisabetta Ranucci, Paolo Ferruti, Michela Matteoli, Amedea Manfredi, Flavia Antonucci

Risultato della ricerca: Articlepeer review

10 Citazioni (Scopus)

Abstract

This paper reports on a novel application of an amphoteric water-soluble polyamidoaminenamed AGMA1 bearing 4-butylguanidine pendants. AGMA1 is an amphoteric, prevailinglycationic polyelectrolyte with isoelectric point of about 10. At pH 7.4 it is zwitterionic with anaverage of 0.55 excess positive charges per unit, notwithstanding it is highly biocompatible. Inthis work, it was found that AGMA1 surface-adsorbed on cell culturing coverslips exhibitsexcellent properties as adhesion and proliferation promoter of primary brain cells such asmicroglia, as well as of hippocampal neurons and astrocytes. Microglia cells cultured onAGMA1-coated coverslips substrate displayed the typical resting, ramified morphology of thosecultured on poly-L-lysine and poly-L-ornithine, employed as reference substrates. Mixedcultures of primary astrocytes and neuronal cells grown on AGMA1- and poly-L-lysine coatedcoverslips were morphologically undistinguishable. On both substrates, neurons differentiatedaxon and dendrites and eventually established perfectly functional synaptic contacts.Quantitative immunocytochemical staining revealed no difference between AGMA1 and poly-Llysine.Electrophysiological experiments allowed recording neuron spontaneous activity onAGMA1. In addition, cell cultures on both AGMA1 and PLL displayed comparable excitatoryand inhibitory neurotransmission, demonstrating that the synaptic contacts formed were fullyfunctional.
Lingua originaleEnglish
pagine (da-a)045007-
Numero di pagine9
RivistaScience and Technology of Advanced Materials
Volume15
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

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