A serum fucose-binding lectin (D1FBL) from adult Didentrarchus labrax is expressed in larva and juvenile tissue and contained in eggs

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11 Citazioni (Scopus)

Abstract

The purification, cloning, sequencing, molecularproperties and expression of a fucose-binding lectin fromthe serum of Dicentrarchus labrax (DlFBL) have beenpreviously reported. We now describe the distribution andexpression of DlFBL during fish ontogeny. Immunohistochemistryand in situ hybridization assays were carried outat various developmental stages (from 10 days posthatchinglarvae to juveniles). Another fucose-bindinglectin, similar to DlFBL in biochemical, immunochemicaland agglutinating properties, was extracted and purifiedfrom eggs and appeared to be localized in the embryo yolksack residual. DlFBL was found in columnar and gobletcells of the intestinal epithelium of larvae (from 20 dayspost-hatching) and juveniles and in parenchymal tissue ofjuveniles. DlFBL mRNA and protein were detected in theintestinal epithelium and in hepatocytes. An amplificationproduct from degenerate primers indicates that lectinisotypes with DlFBL epitopes are expressed in eggs andembryos. Whether the lectin fraction isolated from eggs andembryos includes DlFBL of maternal origin remainsunclear.
Lingua originaleEnglish
pagine (da-a)279-288
Numero di pagine10
RivistaCell and Tissue Research
Volume341
Stato di pubblicazionePublished - 2010

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Eggs
Larva
Serum
Bass
Fucose
Intestinal Mucosa
Lectins
In Situ Hybridization
Organism Cloning
Epitopes
Hepatocytes
Fishes
Embryonic Structures
Epithelium
Mothers
Messenger RNA
fucose-binding lectin
Proteins
Parenchymal Tissue

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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title = "A serum fucose-binding lectin (D1FBL) from adult Didentrarchus labrax is expressed in larva and juvenile tissue and contained in eggs",
abstract = "The purification, cloning, sequencing, molecularproperties and expression of a fucose-binding lectin fromthe serum of Dicentrarchus labrax (DlFBL) have beenpreviously reported. We now describe the distribution andexpression of DlFBL during fish ontogeny. Immunohistochemistryand in situ hybridization assays were carried outat various developmental stages (from 10 days posthatchinglarvae to juveniles). Another fucose-bindinglectin, similar to DlFBL in biochemical, immunochemicaland agglutinating properties, was extracted and purifiedfrom eggs and appeared to be localized in the embryo yolksack residual. DlFBL was found in columnar and gobletcells of the intestinal epithelium of larvae (from 20 dayspost-hatching) and juveniles and in parenchymal tissue ofjuveniles. DlFBL mRNA and protein were detected in theintestinal epithelium and in hepatocytes. An amplificationproduct from degenerate primers indicates that lectinisotypes with DlFBL epitopes are expressed in eggs andembryos. Whether the lectin fraction isolated from eggs andembryos includes DlFBL of maternal origin remainsunclear.",
keywords = "Dicentrarchus labrax",
author = "Aiti Vizzini and Matteo Cammarata and Nicolo' Parrinello and Parisi, {Maria Giovanna} and Giuseppina Salerno and Gigliola Benenati and Valentina Mangano",
year = "2010",
language = "English",
volume = "341",
pages = "279--288",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - A serum fucose-binding lectin (D1FBL) from adult Didentrarchus labrax is expressed in larva and juvenile tissue and contained in eggs

AU - Vizzini, Aiti

AU - Cammarata, Matteo

AU - Parrinello, Nicolo'

AU - Parisi, Maria Giovanna

AU - Salerno, Giuseppina

AU - Benenati, Gigliola

AU - Mangano, Valentina

PY - 2010

Y1 - 2010

N2 - The purification, cloning, sequencing, molecularproperties and expression of a fucose-binding lectin fromthe serum of Dicentrarchus labrax (DlFBL) have beenpreviously reported. We now describe the distribution andexpression of DlFBL during fish ontogeny. Immunohistochemistryand in situ hybridization assays were carried outat various developmental stages (from 10 days posthatchinglarvae to juveniles). Another fucose-bindinglectin, similar to DlFBL in biochemical, immunochemicaland agglutinating properties, was extracted and purifiedfrom eggs and appeared to be localized in the embryo yolksack residual. DlFBL was found in columnar and gobletcells of the intestinal epithelium of larvae (from 20 dayspost-hatching) and juveniles and in parenchymal tissue ofjuveniles. DlFBL mRNA and protein were detected in theintestinal epithelium and in hepatocytes. An amplificationproduct from degenerate primers indicates that lectinisotypes with DlFBL epitopes are expressed in eggs andembryos. Whether the lectin fraction isolated from eggs andembryos includes DlFBL of maternal origin remainsunclear.

AB - The purification, cloning, sequencing, molecularproperties and expression of a fucose-binding lectin fromthe serum of Dicentrarchus labrax (DlFBL) have beenpreviously reported. We now describe the distribution andexpression of DlFBL during fish ontogeny. Immunohistochemistryand in situ hybridization assays were carried outat various developmental stages (from 10 days posthatchinglarvae to juveniles). Another fucose-bindinglectin, similar to DlFBL in biochemical, immunochemicaland agglutinating properties, was extracted and purifiedfrom eggs and appeared to be localized in the embryo yolksack residual. DlFBL was found in columnar and gobletcells of the intestinal epithelium of larvae (from 20 dayspost-hatching) and juveniles and in parenchymal tissue ofjuveniles. DlFBL mRNA and protein were detected in theintestinal epithelium and in hepatocytes. An amplificationproduct from degenerate primers indicates that lectinisotypes with DlFBL epitopes are expressed in eggs andembryos. Whether the lectin fraction isolated from eggs andembryos includes DlFBL of maternal origin remainsunclear.

KW - Dicentrarchus labrax

UR - http://hdl.handle.net/10447/51433

M3 - Article

VL - 341

SP - 279

EP - 288

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

ER -