The aim of this paper is to describe a multidisciplinary approach including biological and particlemonitoring, andmicroclimate analysis associated with the application of the Computational Fluid Dynamic (CFD). This approachwas applied at the Palatina historical library in Parma. Monitoring was performed both in July and in December,in the absence of visitors and operators. Air microbial monitoring was performed with active and passivemethods. Airborne particles with a diameter of !0.3, !0.5, !1 and !5 μm/m3, were counted by a laser particlecounter. The surface contamination of shelves and manuscripts was assessed with nitrocellulose membranes.A spore trap sampler was used to identify both viable and non-viable fungal spores by optical microscope. Microbiologicalcontaminantswere analyzed through cultural andmolecular biology techniques. Microclimatic parameterswere also recorded. An infrared thermal camera provided information on the surface temperature of thedifferent building materials, objects and components. Transient simulation models, for coupled heat and massmoisturetransfer, taking into account archivist and general public movements, combined with the relatedsensible and latent heat released into the environment, were carried out applying the CFD-FE (Finite Elements)method. Simulations of particle tracing were carried out.A wide variability in environmental microbial contamination, both for air and surfaces, was observed.Cladosporium spp., Alternaria spp., Aspergillus spp., and Penicillium spp. were the most frequently foundmicrofungi. Bacteria such as Streptomyces spp., Bacillus spp., Sphingomonas spp., and Pseudoclavibacter as wellas unculturable colonies were characterized by molecular investigation. CFD simulation results obtained wereconsistent with the experimental data on microclimatic conditions. The tracing and distribution of particlesshowed the different slice planes of diffusion mostly influenced by the convective airflow.This interdisciplinary research represents a contribution towards the definition of standardized methods forassessing the biological and microclimatic quality of indoor cultural heritage environments.
|Numero di pagine||11|
|Rivista||Science of the Total Environment|
|Stato di pubblicazione||Published - 2015|
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