Abstract

The knowledge and treatment of alcoholic liver disease is still plagued with gaps mostly due to the inherent limitations of research with patients. We developed an animal model for studying liver histopathology, Hsp-chaperones involvement, and response to treatment. The system was standardized using mice to which ethanol was orally administered alone or in combination with Lactobacillus fermentum for 4, 8 and 12 weeks and applying a battery of techniques (histology, immunohistochemistry, Western blotting, real-time PCR, immunoprecipitation, 3-nitrotyrosine labeling) to assess liver pathology and Hsp60, iNOS gene expression and protein levels, and Hsp60 post-translational modifications. Steatosis score, iNOS levels, and nitrosylated proteins (e.g., Hsp60) decreased after probiotic intake reducing considerably ethanol-induced tissue damage However, one may assume that the probiotic tested has a gut protective effect and, possibly, anti-steatotic and antioxidant effects in the liver. Our results provide novel insights that may be taken into account while devising new approaches for treating liver diseases associated with alcohol consumption.
Lingua originaleEnglish
Pagine99-99
Numero di pagine1
Stato di pubblicazionePublished - 2016

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Lactobacillus fermentum
Alcoholic Liver Diseases
Probiotics
Liver
Ethanol
Chaperonin 60
Post Translational Protein Processing
Immunoprecipitation
Alcohol Drinking
Liver Diseases
Real-Time Polymerase Chain Reaction
Histology
Animal Models
Antioxidants
Western Blotting
Immunohistochemistry
Pathology
Gene Expression
Therapeutics
Research

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title = "A mouse model of alcoholic liver disease reveals protection by Lactobacillus fermentum",
abstract = "The knowledge and treatment of alcoholic liver disease is still plagued with gaps mostly due to the inherent limitations of research with patients. We developed an animal model for studying liver histopathology, Hsp-chaperones involvement, and response to treatment. The system was standardized using mice to which ethanol was orally administered alone or in combination with Lactobacillus fermentum for 4, 8 and 12 weeks and applying a battery of techniques (histology, immunohistochemistry, Western blotting, real-time PCR, immunoprecipitation, 3-nitrotyrosine labeling) to assess liver pathology and Hsp60, iNOS gene expression and protein levels, and Hsp60 post-translational modifications. Steatosis score, iNOS levels, and nitrosylated proteins (e.g., Hsp60) decreased after probiotic intake reducing considerably ethanol-induced tissue damage However, one may assume that the probiotic tested has a gut protective effect and, possibly, anti-steatotic and antioxidant effects in the liver. Our results provide novel insights that may be taken into account while devising new approaches for treating liver diseases associated with alcohol consumption.",
keywords = "Ethanol-induced liver pathology, probiotics, steatosis",
author = "Giovanni Tomasello and {Marino Gammazza}, Antonella and {Caruso Bavisotto}, Celeste and Giovanni Zummo and Felicia Farina and Rosario Barone and Claudia Sangiorgi and {Di Felice}, Valentina and Francesca Rappa and Francesco Cappello",
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T1 - A mouse model of alcoholic liver disease reveals protection by Lactobacillus fermentum

AU - Tomasello, Giovanni

AU - Marino Gammazza, Antonella

AU - Caruso Bavisotto, Celeste

AU - Zummo, Giovanni

AU - Farina, Felicia

AU - Barone, Rosario

AU - Sangiorgi, Claudia

AU - Di Felice, Valentina

AU - Rappa, Francesca

AU - Cappello, Francesco

PY - 2016

Y1 - 2016

N2 - The knowledge and treatment of alcoholic liver disease is still plagued with gaps mostly due to the inherent limitations of research with patients. We developed an animal model for studying liver histopathology, Hsp-chaperones involvement, and response to treatment. The system was standardized using mice to which ethanol was orally administered alone or in combination with Lactobacillus fermentum for 4, 8 and 12 weeks and applying a battery of techniques (histology, immunohistochemistry, Western blotting, real-time PCR, immunoprecipitation, 3-nitrotyrosine labeling) to assess liver pathology and Hsp60, iNOS gene expression and protein levels, and Hsp60 post-translational modifications. Steatosis score, iNOS levels, and nitrosylated proteins (e.g., Hsp60) decreased after probiotic intake reducing considerably ethanol-induced tissue damage However, one may assume that the probiotic tested has a gut protective effect and, possibly, anti-steatotic and antioxidant effects in the liver. Our results provide novel insights that may be taken into account while devising new approaches for treating liver diseases associated with alcohol consumption.

AB - The knowledge and treatment of alcoholic liver disease is still plagued with gaps mostly due to the inherent limitations of research with patients. We developed an animal model for studying liver histopathology, Hsp-chaperones involvement, and response to treatment. The system was standardized using mice to which ethanol was orally administered alone or in combination with Lactobacillus fermentum for 4, 8 and 12 weeks and applying a battery of techniques (histology, immunohistochemistry, Western blotting, real-time PCR, immunoprecipitation, 3-nitrotyrosine labeling) to assess liver pathology and Hsp60, iNOS gene expression and protein levels, and Hsp60 post-translational modifications. Steatosis score, iNOS levels, and nitrosylated proteins (e.g., Hsp60) decreased after probiotic intake reducing considerably ethanol-induced tissue damage However, one may assume that the probiotic tested has a gut protective effect and, possibly, anti-steatotic and antioxidant effects in the liver. Our results provide novel insights that may be taken into account while devising new approaches for treating liver diseases associated with alcohol consumption.

KW - Ethanol-induced liver pathology

KW - probiotics

KW - steatosis

UR - http://hdl.handle.net/10447/202599

M3 - Other

SP - 99

EP - 99

ER -