A Method to Discriminate Between the Candida stellata and Saccharomyces cerevisiae in Mixed Fermentation on WLD and Lysine Agar Media

Diego Planeta, Di Maio, Polizzotto, Oliva

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Abstract

This paper presents a simple method to distinguish between Candida stellata and Saccharomyces cerevisiaeyeasts during microbiological analyses. The method is based on differential yeast growth on a mediumcontaining cycloheximide and a medium containing lysine as only nitrogen source (lysine agar). Thecycloheximide resistance of 45 yeast strains belonging to Candida stellata, Hanseniaspora uvarum, Hanseniasporaguilliermondii, Metschnikowia pulcherrima, Torulaspora delbrueckii, Zygosaccharomyces bailii, Kluyveromycesthermotolerans and Zygoascus hellenicus, and 14 strains of Saccharomyces cerevisiae and Saccharomycesbayanus on WL nutrient agar, was assayed. Cycloheximide resistance is characteristic of the species H. uvarum,H. guilliermondii and Z. hellenicus, while for the other yeasts it depends on the strain and the concentrationof cycloheximide used. Two mg/L of cycloheximide allows selective counting of a strain of C. stellata (Cs3)compared to one of the sensitive S. cerevisiae strain (NDA21). Similar results can be obtained on lysine agar,but counts are reliable only with the additional spreading of a monolayer of Saccharomyces cells. The differentcycloheximide resistance of C. stellata and S. cerevisiae can be used in the microbiological analysis of mixedcultures to monitor the individual growth of the two yeast species. This method can be applied to the studyof mixed fermentations with other non-Saccharomyces species. The modified use of lysine agar is useful to acertain extent in the distinction of multistarter yeasts from the indigenous yeasts.
Lingua originaleEnglish
pagine (da-a)35-41
Numero di pagine7
RivistaSOUTH AFRICAN JOURNAL FOR ENOLOGY AND VITICULTURE
VolumeVol. 32, No. 1, 2011
Stato di pubblicazionePublished - 2011

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All Science Journal Classification (ASJC) codes

  • Food Science
  • Horticulture

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