A methacrylic hyaluronic acid derivative for potential application in oral treatment of celiac disease

Fabio Salvatore Palumbo, Gaetano Giammona, Giovanna Pitarresi, Daniela Triolo, Calogero Fiorica, Calogero Fiorica, Daniela Triolo, Fabio Salvatore Palumbo, Gaetano Giammona

Risultato della ricerca: Articlepeer review

Abstract

Objective: Aim of this work was the synthesis of a methacrylic hyaluronic acid (HA) derivative and the production, via photocrosslinking, of related hydrogels loaded with an endopeptidase intended for a potential oral treatment of celiac disease. Methods: The methacrylic derivative of HA was prepared through a one-pot procedure involving the reaction with ethylenediamine (EDA) and methacrylic anhydride (MA). The obtained derivative, named HA-EDA-MA, was used to prepare photocrosslinked hydrogels loaded with a prolyl endopeptidase derived from Flavobacterium meningosepticum (PEP FM) able to detoxify gliadin. Obtained hydrogels were recovered as gels or freeze-dried powders. Results: Hydrogels obtained as freeze-dried powders, are able to protect loaded enzyme from degradation due to freeze-drying process and from alteration during storage, overall in the presence of a cryoprotectant. All photocrosslinked HA-EDA-MA hydrogels (gels and powders) release PEP FM in simulated intestinal fluid in sustained manner and in active form. HA-EDA-MA hydrogels are nontoxic as demonstrated through in vitro studies on BALB 3T3 cells. Conclusions: Prepared hydrogels show a potential application for oral treatment of celiac disease thanks to the possibility to release enzymes able to detoxify the gliadin peptide that induces the immunogenic response.
Lingua originaleEnglish
pagine (da-a)1480-1488
Numero di pagine9
RivistaDrug Development and Industrial Pharmacy
Volume43
Stato di pubblicazionePublished - 2017

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

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