Abstract

The aim of the present study was to evaluate the expression of the heat shock protein 60 (HSP60), a mitochondrial matrix-associated protein belonging to the chaperonin family, in colorectal adenomas and cancers, comparing them to normal colonic tissues and hyperplastic polyps. We performed both immunohistochemistry and Western blot analysis for HSP60. Immunohistochemistry resulted positive in all tubular adenomas and infiltrating adenocarcinomas. By contrast, normal tissues and hyperplastic polyps were negative. Quantitative analysis showed that tubular adenomas with different levels of dysplasia did not present statistical differences concerning HSP60 positivity. In addition, carcinomas always showed the highest expression. Western blot analysis confirmed these observations. These data suggest that HSP60 over-expression is an early event in carcinogenesis. We suspect that HSP60 plays a different role in colorectal carcinogenesis with respect to that in normal cells, which foresees its possible use as diagnostic and prognostic tools.
Lingua originaleEnglish
pagine (da-a)105-109
Numero di pagine5
RivistaEUROPEAN JOURNAL OF HISTOCHEMISTRY
Volume47
Stato di pubblicazionePublished - 2003

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Chaperonins
Chaperonin 60
chaperonins
heat shock proteins
carcinogenesis
Carcinogenesis
adenoma
Adenoma
Polyps
immunohistochemistry
Western blotting
Western Blotting
Immunohistochemistry
adenocarcinoma
carcinoma
quantitative analysis
Colorectal Neoplasms
Adenocarcinoma
Carcinoma
neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Histology
  • Cell Biology

Cita questo

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title = "60KDa chaperonin (HSP60) is over-expressed during colorectal carcinogenesis",
abstract = "The aim of the present study was to evaluate the expression of the heat shock protein 60 (HSP60), a mitochondrial matrix-associated protein belonging to the chaperonin family, in colorectal adenomas and cancers, comparing them to normal colonic tissues and hyperplastic polyps. We performed both immunohistochemistry and Western blot analysis for HSP60. Immunohistochemistry resulted positive in all tubular adenomas and infiltrating adenocarcinomas. By contrast, normal tissues and hyperplastic polyps were negative. Quantitative analysis showed that tubular adenomas with different levels of dysplasia did not present statistical differences concerning HSP60 positivity. In addition, carcinomas always showed the highest expression. Western blot analysis confirmed these observations. These data suggest that HSP60 over-expression is an early event in carcinogenesis. We suspect that HSP60 plays a different role in colorectal carcinogenesis with respect to that in normal cells, which foresees its possible use as diagnostic and prognostic tools.",
author = "Marianna Bellafiore and Francesco Cappello and Giuseppe Modica and Carmelo Sciume' and Antonio Palma and Felicia Farina and Fabio Bucchieri and Sabrina David and Giovanni Zummo and Francesco Cappello and Vito Marciano'",
year = "2003",
language = "English",
volume = "47",
pages = "105--109",
journal = "EUROPEAN JOURNAL OF HISTOCHEMISTRY",
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TY - JOUR

T1 - 60KDa chaperonin (HSP60) is over-expressed during colorectal carcinogenesis

AU - Bellafiore, Marianna

AU - Cappello, Francesco

AU - Modica, Giuseppe

AU - Sciume', Carmelo

AU - Palma, Antonio

AU - Farina, Felicia

AU - Bucchieri, Fabio

AU - David, Sabrina

AU - Zummo, Giovanni

AU - Cappello, Francesco

AU - Marciano', Vito

PY - 2003

Y1 - 2003

N2 - The aim of the present study was to evaluate the expression of the heat shock protein 60 (HSP60), a mitochondrial matrix-associated protein belonging to the chaperonin family, in colorectal adenomas and cancers, comparing them to normal colonic tissues and hyperplastic polyps. We performed both immunohistochemistry and Western blot analysis for HSP60. Immunohistochemistry resulted positive in all tubular adenomas and infiltrating adenocarcinomas. By contrast, normal tissues and hyperplastic polyps were negative. Quantitative analysis showed that tubular adenomas with different levels of dysplasia did not present statistical differences concerning HSP60 positivity. In addition, carcinomas always showed the highest expression. Western blot analysis confirmed these observations. These data suggest that HSP60 over-expression is an early event in carcinogenesis. We suspect that HSP60 plays a different role in colorectal carcinogenesis with respect to that in normal cells, which foresees its possible use as diagnostic and prognostic tools.

AB - The aim of the present study was to evaluate the expression of the heat shock protein 60 (HSP60), a mitochondrial matrix-associated protein belonging to the chaperonin family, in colorectal adenomas and cancers, comparing them to normal colonic tissues and hyperplastic polyps. We performed both immunohistochemistry and Western blot analysis for HSP60. Immunohistochemistry resulted positive in all tubular adenomas and infiltrating adenocarcinomas. By contrast, normal tissues and hyperplastic polyps were negative. Quantitative analysis showed that tubular adenomas with different levels of dysplasia did not present statistical differences concerning HSP60 positivity. In addition, carcinomas always showed the highest expression. Western blot analysis confirmed these observations. These data suggest that HSP60 over-expression is an early event in carcinogenesis. We suspect that HSP60 plays a different role in colorectal carcinogenesis with respect to that in normal cells, which foresees its possible use as diagnostic and prognostic tools.

UR - http://hdl.handle.net/10447/191074

M3 - Article

VL - 47

SP - 105

EP - 109

JO - EUROPEAN JOURNAL OF HISTOCHEMISTRY

JF - EUROPEAN JOURNAL OF HISTOCHEMISTRY

SN - 1121-760X

ER -