12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products

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3 Citazioni (Scopus)

Abstract

For a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairyproducts, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheesesof Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovisaries) mitochondrial species-specific gene fragment. The use of species-specific primers for Bos taurus, Caprahircus and Ovis aries species, after electrophoresis on agarose gel, yielded fragments of 256 bp, 326 bp and172 bp, respectively. Amplification by duplex-PCR of DNA pools from two species showed detection thresholdsof 0.1% of “contaminant” DNA in each mixture. Finally, duplex-PCR assay was applied to experimental cheesesin order to detect the minimum threshold of DNA belonging to one species in cheese made with milk of twospecies. The results showed a sensitive threshold of 0.1% of ewes' milk in cows' and goats' cheeses, 0.1% of cows'milk in ewes' and goats' cheeses, and finally 0.1% of goats' milk in cows' and ewes' cheeses. The proposed assayrepresents a rapid and straightforward method of species traceability for the detections of adulteration inSicilian mono-species dairy products.
Lingua originaleEnglish
pagine (da-a)39-44
Numero di pagine6
RivistaDefault journal
Volume193
Stato di pubblicazionePublished - 2016

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Mitochondrial Genes
Dairy Products
rRNA Genes
dairy products
Cheese
Milk
Goats
ribosomal RNA
goat cheese
ewe milk
goat milk
DNA
cheeses
ewes
genes
dairy cows
Polymerase Chain Reaction
milk
traceability
Domestic Sheep

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • veterinary(all)

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title = "12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products",
abstract = "For a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairyproducts, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheesesof Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovisaries) mitochondrial species-specific gene fragment. The use of species-specific primers for Bos taurus, Caprahircus and Ovis aries species, after electrophoresis on agarose gel, yielded fragments of 256 bp, 326 bp and172 bp, respectively. Amplification by duplex-PCR of DNA pools from two species showed detection thresholdsof 0.1{\%} of “contaminant” DNA in each mixture. Finally, duplex-PCR assay was applied to experimental cheesesin order to detect the minimum threshold of DNA belonging to one species in cheese made with milk of twospecies. The results showed a sensitive threshold of 0.1{\%} of ewes' milk in cows' and goats' cheeses, 0.1{\%} of cows'milk in ewes' and goats' cheeses, and finally 0.1{\%} of goats' milk in cows' and ewes' cheeses. The proposed assayrepresents a rapid and straightforward method of species traceability for the detections of adulteration inSicilian mono-species dairy products.",
author = "Sardina, {Maria Teresa} and {Di Gerlando}, Rosalia and Lina Tortorici and Salvatore Mastrangelo and Marco Tolone",
year = "2016",
language = "English",
volume = "193",
pages = "39--44",
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TY - JOUR

T1 - 12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products

AU - Sardina, Maria Teresa

AU - Di Gerlando, Rosalia

AU - Tortorici, Lina

AU - Mastrangelo, Salvatore

AU - Tolone, Marco

PY - 2016

Y1 - 2016

N2 - For a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairyproducts, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheesesof Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovisaries) mitochondrial species-specific gene fragment. The use of species-specific primers for Bos taurus, Caprahircus and Ovis aries species, after electrophoresis on agarose gel, yielded fragments of 256 bp, 326 bp and172 bp, respectively. Amplification by duplex-PCR of DNA pools from two species showed detection thresholdsof 0.1% of “contaminant” DNA in each mixture. Finally, duplex-PCR assay was applied to experimental cheesesin order to detect the minimum threshold of DNA belonging to one species in cheese made with milk of twospecies. The results showed a sensitive threshold of 0.1% of ewes' milk in cows' and goats' cheeses, 0.1% of cows'milk in ewes' and goats' cheeses, and finally 0.1% of goats' milk in cows' and ewes' cheeses. The proposed assayrepresents a rapid and straightforward method of species traceability for the detections of adulteration inSicilian mono-species dairy products.

AB - For a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairyproducts, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheesesof Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovisaries) mitochondrial species-specific gene fragment. The use of species-specific primers for Bos taurus, Caprahircus and Ovis aries species, after electrophoresis on agarose gel, yielded fragments of 256 bp, 326 bp and172 bp, respectively. Amplification by duplex-PCR of DNA pools from two species showed detection thresholdsof 0.1% of “contaminant” DNA in each mixture. Finally, duplex-PCR assay was applied to experimental cheesesin order to detect the minimum threshold of DNA belonging to one species in cheese made with milk of twospecies. The results showed a sensitive threshold of 0.1% of ewes' milk in cows' and goats' cheeses, 0.1% of cows'milk in ewes' and goats' cheeses, and finally 0.1% of goats' milk in cows' and ewes' cheeses. The proposed assayrepresents a rapid and straightforward method of species traceability for the detections of adulteration inSicilian mono-species dairy products.

UR - http://hdl.handle.net/10447/208212

M3 - Article

VL - 193

SP - 39

EP - 44

JO - Default journal

JF - Default journal

ER -