[automatically translated] Since the early days of the circulation of the pandemic virus H1N1-2009 in the population, it was reported transmission of the virus from man to different animal species (pig, turkey, dog, cat). The transmission of influenza virus from humans to animals is not only a problem confined to the livestock sector, but a problem of public health for the role of "" reservoir of influenza viruses represented by these species. The pig has so far proven an important epidemiological role in the spread of A (H1N1) pdm man; However, this does not exclude, that as a result of viral evolution, or reassortment phenomena, from that species emerge and spread to man viruses with different and / or higher pathogenicity. The identification, in the short term, the new circulating influenza strains in pigs so is the cornerstone of surveillance for influenza viruses; to this end, a system has been optimized for the rapid whole genome sequencing of H1N1pdm viruses, the results of which are reported in the work. In Italy, the first case of infection with A (H1N1) pdm in pigs was detected in breeding Nervino (Lombardy) in December 2009; until August 2010 were isolated n ° 8 H1N1pdm virus in pigs raised in Lombardy and Sicily. After replication of the isolates in embryonated and / or egg cell cultures (MDCK and CACO-2), the ANNv was extracted using the Viral RNA Mini Kit QIAamp® kit; They were then set up in n. 46 one-step RT-PCR with specific primers for amplification of individual gene segments of different length. The system provides that the amplification stages, loading in gel electrophoresis, purification and marking, they are carried out in plates 96x, in order to speed up the operations of the laboratory. The analysis of sequences and phylogeny of 8 viral isolates has allowed to characterize them as H1N1pdm virus, with high percentage homology (up to 100%), for all the gene segments, with H1N1pdm viruses circulating in the human population. The activation of virological surveillance in pig farms also allowed, for the first time in Europe, the isolation of a "new" reassortant viruses (A / H1N2), characterized by 7 genes derived from viruses H1N1pdm and Neuraminidase gene ( N2) derived from H1N2 swine flu viruses isolated recently in Italy and Sweden. The cases of "reverse zoonosis" reported, highlight, once again, the '
|Number of pages||1|
|Publication status||Published - 2011|