Valutazione metodologica della contaminazione microbica dell’aria in sala operatoria: campionamento attivo e campionamento passivo

[automatically translated] OBJECTIVES: Evaluation of microbiological quality of air in operating rooms dell'AOUP "P. Giaccone "of Palermo using active and passive sampling methods. MATERIALS: In the second half of 2009 were performed air sampling in 20 operating rooms (10 and 10 laminar flow to turbulent flow) in a state of activity is at rest. The sampling points for the active sampling were: 1) the center of the room 2) the VCCC; for passive sampling 1) freestanding 2) back room. The following parameters were monitored: total bacterial count (CBT), the total charge mycetic (CMT), Staphylococcus aureus and for bacteria and fungi Microbial Air Index (IMA). The isolated microorganisms were subject to identification. The data obtained were processed using EpiInfo 3.5.1. SUMMARY: The active sampling found CBT amounted to 87.7 ± 65.7 CFU / m3 and CMT of 0.8 ± 1.5 CFU / m3 in the center of the room while in VCCC were detected CBT amounting to 16.5 ± 22, 2 CFU / m3 and CMT of 0.8 ± 1.1 CFU / m3. The passive sampling during surgical activities showed IMA values of 19.8 ± 13.5 to 0.1 ± 0.3 for bacteria and fungi in the center of the room, while in the bottom of the room IMA values of 16.7 ± 13 bacteria and 0.6 ± 1 for mycetes. In the absence of activity, you are recognized IMA values of 3.9 ± 3.9 to 0.1 ± 0.2 for bacteria and fungi in the center of the room while the IMA values room fund of 2.4 ± 3.1 bacteria and 0.2 ± 0.4 for fungi. In no sample it was found to contain S. aureus. The isolated mold, with a higher frequency in active sampling, were Cephalosporium spp, Aspergillus spp and Pennicillum spp. The processing of the data showed that only bacterial counts detected with both sampling methods were significantly correlated with each other (p <0.001). CONCLUSIONS: Although there are no clear indications relating to air sampling methods, for the limits that the various methods pose, the correlation between the CBT and IMA values for bacteria suggests the adoption of the two alternative methods for the assessment of bacterial load .