VALIDATION OF A MODIFIED MODEL OF TNBS-INDUCED COLITIS IN RATS. HOW TO INDUCE A CHEMICAL COLITIS IN RATS.

Salvatore Buscemi, Attilio Ignazio Lo Monte, Giovanni Tomasello, Alida Abruzzo, Francesco Damiani, Emanuele Sinagra, Vincenzo Davide Palumbo, Mario Cottone, Dario Raimondo, Emanuele Sinagra, Roberto Puleio, Giovanni Tomasello, Vincenzo Davide Palumbo, Giovanna Traina

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2 Citations (Scopus)

Abstract

Background: there are no standard practice in the induction of colitis by 2,4,6-trinitrobenzene sulfonic (TNBS) acid. Usually, the repeated administration of TNBS is preferred, because it will result in a local Th1 response that has the characteristics of Crohn's disease. material and Methods: A total of 30 rats were randomized into two groups, consisting of a saline control group of ten rats and a TNBS groups of 20 rats. After the animals were anesthesized, 0,5 ml of either 0,9 % saline 8controls) or TNBS 50 mg/Kg dissolved in 50% ethanol were instilled into the colon through a rubber catheter. The experiment was repeated weekly for four weeks, then, the rats were killed at day 40, and the distal colon removed. results: At day 40, the bowel wall basically normal in the control group. In the TNBS group, the bowel lumen became narrow with tickened wall, and the mucosal surface presented adherent membrane with brown black, linear ulcers, proliferous lymphocites tissue, inflammatory granulomas and submucosal neutrophil infiltration. The median score of the severity of the colonic damage was 0 in the control group, and 4,75 (range 4-5) in the TNBS group; the mean weight of the rats was 180+35 g in the TNBS group, while it was 215+25 in the control group. Conclusions: The presented experiment is a cost-effective and safe method to induce Crohn-like colonic damage using a lower dose of TNBS, thus avoiding the risk of a massive loss of rats. This model is rather suitable for the assessment of the effects of potential therapeutic agent.
Original languageEnglish
Pages (from-to)92-96
Number of pages5
JournalACTA BIO-MEDICA DE L'ATENEO PARMENSE
Volume86
Publication statusPublished - 2015

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Colitis
Control Groups
Colon
Sulfonic Acids
Neutrophil Infiltration
sym-trinitrobenzene
Rubber
Therapeutic Uses
Granuloma
Crohn Disease
Ulcer
Ethanol
Catheters
Weights and Measures
Costs and Cost Analysis
Membranes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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VALIDATION OF A MODIFIED MODEL OF TNBS-INDUCED COLITIS IN RATS. HOW TO INDUCE A CHEMICAL COLITIS IN RATS. / Buscemi, Salvatore; Lo Monte, Attilio Ignazio; Tomasello, Giovanni; Abruzzo, Alida; Damiani, Francesco; Sinagra, Emanuele; Palumbo, Vincenzo Davide; Cottone, Mario; Raimondo, Dario; Sinagra, Emanuele; Puleio, Roberto; Tomasello, Giovanni; Palumbo, Vincenzo Davide; Traina, Giovanna.

In: ACTA BIO-MEDICA DE L'ATENEO PARMENSE, Vol. 86, 2015, p. 92-96.

Research output: Contribution to journalArticle

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title = "VALIDATION OF A MODIFIED MODEL OF TNBS-INDUCED COLITIS IN RATS. HOW TO INDUCE A CHEMICAL COLITIS IN RATS.",
abstract = "Background: there are no standard practice in the induction of colitis by 2,4,6-trinitrobenzene sulfonic (TNBS) acid. Usually, the repeated administration of TNBS is preferred, because it will result in a local Th1 response that has the characteristics of Crohn's disease. material and Methods: A total of 30 rats were randomized into two groups, consisting of a saline control group of ten rats and a TNBS groups of 20 rats. After the animals were anesthesized, 0,5 ml of either 0,9 {\%} saline 8controls) or TNBS 50 mg/Kg dissolved in 50{\%} ethanol were instilled into the colon through a rubber catheter. The experiment was repeated weekly for four weeks, then, the rats were killed at day 40, and the distal colon removed. results: At day 40, the bowel wall basically normal in the control group. In the TNBS group, the bowel lumen became narrow with tickened wall, and the mucosal surface presented adherent membrane with brown black, linear ulcers, proliferous lymphocites tissue, inflammatory granulomas and submucosal neutrophil infiltration. The median score of the severity of the colonic damage was 0 in the control group, and 4,75 (range 4-5) in the TNBS group; the mean weight of the rats was 180+35 g in the TNBS group, while it was 215+25 in the control group. Conclusions: The presented experiment is a cost-effective and safe method to induce Crohn-like colonic damage using a lower dose of TNBS, thus avoiding the risk of a massive loss of rats. This model is rather suitable for the assessment of the effects of potential therapeutic agent.",
author = "Salvatore Buscemi and {Lo Monte}, {Attilio Ignazio} and Giovanni Tomasello and Alida Abruzzo and Francesco Damiani and Emanuele Sinagra and Palumbo, {Vincenzo Davide} and Mario Cottone and Dario Raimondo and Emanuele Sinagra and Roberto Puleio and Giovanni Tomasello and Palumbo, {Vincenzo Davide} and Giovanna Traina",
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AU - Buscemi, Salvatore

AU - Lo Monte, Attilio Ignazio

AU - Tomasello, Giovanni

AU - Abruzzo, Alida

AU - Damiani, Francesco

AU - Sinagra, Emanuele

AU - Palumbo, Vincenzo Davide

AU - Cottone, Mario

AU - Raimondo, Dario

AU - Sinagra, Emanuele

AU - Puleio, Roberto

AU - Tomasello, Giovanni

AU - Palumbo, Vincenzo Davide

AU - Traina, Giovanna

PY - 2015

Y1 - 2015

N2 - Background: there are no standard practice in the induction of colitis by 2,4,6-trinitrobenzene sulfonic (TNBS) acid. Usually, the repeated administration of TNBS is preferred, because it will result in a local Th1 response that has the characteristics of Crohn's disease. material and Methods: A total of 30 rats were randomized into two groups, consisting of a saline control group of ten rats and a TNBS groups of 20 rats. After the animals were anesthesized, 0,5 ml of either 0,9 % saline 8controls) or TNBS 50 mg/Kg dissolved in 50% ethanol were instilled into the colon through a rubber catheter. The experiment was repeated weekly for four weeks, then, the rats were killed at day 40, and the distal colon removed. results: At day 40, the bowel wall basically normal in the control group. In the TNBS group, the bowel lumen became narrow with tickened wall, and the mucosal surface presented adherent membrane with brown black, linear ulcers, proliferous lymphocites tissue, inflammatory granulomas and submucosal neutrophil infiltration. The median score of the severity of the colonic damage was 0 in the control group, and 4,75 (range 4-5) in the TNBS group; the mean weight of the rats was 180+35 g in the TNBS group, while it was 215+25 in the control group. Conclusions: The presented experiment is a cost-effective and safe method to induce Crohn-like colonic damage using a lower dose of TNBS, thus avoiding the risk of a massive loss of rats. This model is rather suitable for the assessment of the effects of potential therapeutic agent.

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