VALIDATION OF A MODEL OF TNBS-INDUCED COLITIS IN RATS. HOW TO INDUCE COLITIS IN RATS.

Attilio Ignazio Lo Monte, Giovanni Tomasello, Emanuele Sinagra, Vincenzo Davide Palumbo, Alida Abruzzo, Francesco Damiani, Salvatore Buscemi, Giovanna Traina, Dario Raimondo, Emanuele Sinagra, Roberto Puleio, Giovanni Tomasello

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: There is no standard practice in the induction of colitis by 2,4,6-trinitrobenzene sulfonic (TNBS) acid. Usually, the repeated administration of TNBS is preferred, because it will result in a local Th1 response that has the characteristics of Crohn`s disease. Materials and Methods: A total of 30 rats were randomized into two groups, consisting of a saline control group of ten rats and a TNBS groups of 20 rats. After the animals were anesthetized, 0.5 ml of either 0.9% saline (controls) or TNBS 50 mg/kg dissolved in 50% ethanol were instilled into the colon through a rubber catheter. The experiment was repeated weekly for four weeks, then, the rats were killed at day 40, and the distal colon removed. Results: At day 40, the bowel wall was basically normal in the control group. In the TNBS group, the bowel lumen became narrow with thickened wall, and the mucosal surface presented adherent membrane with brown black, linear ulcers, proliferous lymphocyte tissue, inflammatory granulomas and submucosal neutrophil infiltration. The median score of the severity of the colonic damage was 0 in the control group, and 4,75 (range 4-5) in the TNBS group; the mean weight of the rats was 180+35 g in the TNBS group, while it was 215+25 in the control group. Conclusions: The presented experiment is a cost-effective and safe method to induce Crohn-like colonic damage using a lower dose of TNBS, thus avoiding the risk of a massive loss of rats. This model is rather suitable for the assessment of the effects of potential therapeutic agents.
Original languageEnglish
Pages (from-to)92-96
Number of pages5
JournalACTA BIO-MEDICA DE L'ATENEO PARMENSE
Volume86
Publication statusPublished - 2015

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Colitis
Control Groups
Colon
Sulfonic Acids
Neutrophil Infiltration
sym-trinitrobenzene
Rubber
Therapeutic Uses
Granuloma
Crohn Disease
Ulcer
Ethanol
Catheters
Lymphocytes
Weights and Measures
Costs and Cost Analysis
Membranes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

VALIDATION OF A MODEL OF TNBS-INDUCED COLITIS IN RATS. HOW TO INDUCE COLITIS IN RATS. / Lo Monte, Attilio Ignazio; Tomasello, Giovanni; Sinagra, Emanuele; Palumbo, Vincenzo Davide; Abruzzo, Alida; Damiani, Francesco; Buscemi, Salvatore; Traina, Giovanna; Raimondo, Dario; Sinagra, Emanuele; Puleio, Roberto; Tomasello, Giovanni.

In: ACTA BIO-MEDICA DE L'ATENEO PARMENSE, Vol. 86, 2015, p. 92-96.

Research output: Contribution to journalArticle

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title = "VALIDATION OF A MODEL OF TNBS-INDUCED COLITIS IN RATS. HOW TO INDUCE COLITIS IN RATS.",
abstract = "Background: There is no standard practice in the induction of colitis by 2,4,6-trinitrobenzene sulfonic (TNBS) acid. Usually, the repeated administration of TNBS is preferred, because it will result in a local Th1 response that has the characteristics of Crohn`s disease. Materials and Methods: A total of 30 rats were randomized into two groups, consisting of a saline control group of ten rats and a TNBS groups of 20 rats. After the animals were anesthetized, 0.5 ml of either 0.9{\%} saline (controls) or TNBS 50 mg/kg dissolved in 50{\%} ethanol were instilled into the colon through a rubber catheter. The experiment was repeated weekly for four weeks, then, the rats were killed at day 40, and the distal colon removed. Results: At day 40, the bowel wall was basically normal in the control group. In the TNBS group, the bowel lumen became narrow with thickened wall, and the mucosal surface presented adherent membrane with brown black, linear ulcers, proliferous lymphocyte tissue, inflammatory granulomas and submucosal neutrophil infiltration. The median score of the severity of the colonic damage was 0 in the control group, and 4,75 (range 4-5) in the TNBS group; the mean weight of the rats was 180+35 g in the TNBS group, while it was 215+25 in the control group. Conclusions: The presented experiment is a cost-effective and safe method to induce Crohn-like colonic damage using a lower dose of TNBS, thus avoiding the risk of a massive loss of rats. This model is rather suitable for the assessment of the effects of potential therapeutic agents.",
author = "{Lo Monte}, {Attilio Ignazio} and Giovanni Tomasello and Emanuele Sinagra and Palumbo, {Vincenzo Davide} and Alida Abruzzo and Francesco Damiani and Salvatore Buscemi and Giovanna Traina and Dario Raimondo and Emanuele Sinagra and Roberto Puleio and Giovanni Tomasello",
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AU - Lo Monte, Attilio Ignazio

AU - Tomasello, Giovanni

AU - Sinagra, Emanuele

AU - Palumbo, Vincenzo Davide

AU - Abruzzo, Alida

AU - Damiani, Francesco

AU - Buscemi, Salvatore

AU - Traina, Giovanna

AU - Raimondo, Dario

AU - Sinagra, Emanuele

AU - Puleio, Roberto

AU - Tomasello, Giovanni

PY - 2015

Y1 - 2015

N2 - Background: There is no standard practice in the induction of colitis by 2,4,6-trinitrobenzene sulfonic (TNBS) acid. Usually, the repeated administration of TNBS is preferred, because it will result in a local Th1 response that has the characteristics of Crohn`s disease. Materials and Methods: A total of 30 rats were randomized into two groups, consisting of a saline control group of ten rats and a TNBS groups of 20 rats. After the animals were anesthetized, 0.5 ml of either 0.9% saline (controls) or TNBS 50 mg/kg dissolved in 50% ethanol were instilled into the colon through a rubber catheter. The experiment was repeated weekly for four weeks, then, the rats were killed at day 40, and the distal colon removed. Results: At day 40, the bowel wall was basically normal in the control group. In the TNBS group, the bowel lumen became narrow with thickened wall, and the mucosal surface presented adherent membrane with brown black, linear ulcers, proliferous lymphocyte tissue, inflammatory granulomas and submucosal neutrophil infiltration. The median score of the severity of the colonic damage was 0 in the control group, and 4,75 (range 4-5) in the TNBS group; the mean weight of the rats was 180+35 g in the TNBS group, while it was 215+25 in the control group. Conclusions: The presented experiment is a cost-effective and safe method to induce Crohn-like colonic damage using a lower dose of TNBS, thus avoiding the risk of a massive loss of rats. This model is rather suitable for the assessment of the effects of potential therapeutic agents.

AB - Background: There is no standard practice in the induction of colitis by 2,4,6-trinitrobenzene sulfonic (TNBS) acid. Usually, the repeated administration of TNBS is preferred, because it will result in a local Th1 response that has the characteristics of Crohn`s disease. Materials and Methods: A total of 30 rats were randomized into two groups, consisting of a saline control group of ten rats and a TNBS groups of 20 rats. After the animals were anesthetized, 0.5 ml of either 0.9% saline (controls) or TNBS 50 mg/kg dissolved in 50% ethanol were instilled into the colon through a rubber catheter. The experiment was repeated weekly for four weeks, then, the rats were killed at day 40, and the distal colon removed. Results: At day 40, the bowel wall was basically normal in the control group. In the TNBS group, the bowel lumen became narrow with thickened wall, and the mucosal surface presented adherent membrane with brown black, linear ulcers, proliferous lymphocyte tissue, inflammatory granulomas and submucosal neutrophil infiltration. The median score of the severity of the colonic damage was 0 in the control group, and 4,75 (range 4-5) in the TNBS group; the mean weight of the rats was 180+35 g in the TNBS group, while it was 215+25 in the control group. Conclusions: The presented experiment is a cost-effective and safe method to induce Crohn-like colonic damage using a lower dose of TNBS, thus avoiding the risk of a massive loss of rats. This model is rather suitable for the assessment of the effects of potential therapeutic agents.

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