Caper (Capparis spinosa L.) is a xerophytic shrub cultivated for its fower buds and fruits, used as foodand for their medicinal properties. Breeding programs and even proper taxonomic classifcation of thegenus Capparis has been hampered so far by the lack of reliable genetic information and molecularmarkers. Here, we present the frst genomic resource for C. spinosa, generated by transcriptomicapproach and de novo assembly. The sequencing efort produced nearly 80million clean readsassembled into 124,723 unitranscripts. Careful annotation and comparison with public databasesrevealed homologs to genes with a key role in important metabolic pathways linked to abiotic stresstolerance and bio-compounds production, such purine, thiamine and phenylpropanoid biosynthesis,α-linolenic acid and lipid metabolism. Additionally, a panel of genes involved in stomatal development/distribution and encoding for Stress Associated Proteins (SAPs) was also identifed. We also used thetranscriptomic data to uncover novel molecular markers for caper. Out of 50 SSRs tested, 14 provedpolymorphic and represent the frst set of SSR markers for the genus Capparis. This transcriptome willbe an important contribution to future studies and breeding programs for this orphan crop, aiding to thedevelopment of improved varieties to sustain agriculture in arid conditions.
|Number of pages||16|
|Publication status||Published - 2019|