Temperature and pressure dependence of quercetin-3-O-palmitate interaction with a model phospholipid membrane: film balance and scanning probe microscopy study

Francesco Castelli, Bruno Giuseppe Pignataro, Laura Sardone, Maria G. Sarpietro, Bruno Pignataro, Giovanni Marletta, Francesco Castelli, Giovanni Nicolosi

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Abstract

The molecular interaction of quercetin-3-O-palmitate (QP) with dimyristoylphosphatidylcholine (DMPC) has been studied. Film balancemeasurements of the average molecular area vs QP molar fraction in DMPC/QP mixed monolayers showed that relevant positive deviationsfrom ideality, i.e., a less dense monolayer packing, occurred for a temperature of 10◦C, below the critical melting transition temperature ofDMPC monolayers (Tcm≈20◦C), while ideal behavior was observed at 37◦C, above this phase transition temperature. The positive deviationobserved at low temperatures in the average molecular area increased with the surface pressure. Scanning probe microscopy measurementsperformed on mixed monolayers transferred on mica showed that the deviations from ideality were connected to the formation of nanometric-scale QP-rich domains. However, the formation of aggregates was observed only for relatively high-QP molar fractionsXQP0.25 at 10◦C,while it was not observed at 37◦C, i.e., when the ideal mixing was found at the air/water interface. The observed effects are explained in termsof a temperature- and surface pressure-dependent phase-separation process based on the predominance at low temperature and low molecularmobility of QP–QP and DMPC–DMPC aggregation forces, prompting the formation of QP-rich domains embedded in a DMPC-rich matrix.High temperature prompts the QP/DMPC ideal mixing.
Original languageEnglish
Pages (from-to)329-335
Number of pages7
JournalJournal of Colloid and Interface Science
Volume271
Publication statusPublished - 2004

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Colloid and Surface Chemistry
  • Surfaces, Coatings and Films
  • Biomaterials

Cite this

Temperature and pressure dependence of quercetin-3-O-palmitate interaction with a model phospholipid membrane: film balance and scanning probe microscopy study. / Castelli, Francesco; Pignataro, Bruno Giuseppe; Sardone, Laura; Sarpietro, Maria G.; Pignataro, Bruno; Marletta, Giovanni; Castelli, Francesco; Nicolosi, Giovanni.

In: Journal of Colloid and Interface Science, Vol. 271, 2004, p. 329-335.

Research output: Contribution to journalArticle

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abstract = "The molecular interaction of quercetin-3-O-palmitate (QP) with dimyristoylphosphatidylcholine (DMPC) has been studied. Film balancemeasurements of the average molecular area vs QP molar fraction in DMPC/QP mixed monolayers showed that relevant positive deviationsfrom ideality, i.e., a less dense monolayer packing, occurred for a temperature of 10◦C, below the critical melting transition temperature ofDMPC monolayers (Tcm≈20◦C), while ideal behavior was observed at 37◦C, above this phase transition temperature. The positive deviationobserved at low temperatures in the average molecular area increased with the surface pressure. Scanning probe microscopy measurementsperformed on mixed monolayers transferred on mica showed that the deviations from ideality were connected to the formation of nanometric-scale QP-rich domains. However, the formation of aggregates was observed only for relatively high-QP molar fractionsXQP0.25 at 10◦C,while it was not observed at 37◦C, i.e., when the ideal mixing was found at the air/water interface. The observed effects are explained in termsof a temperature- and surface pressure-dependent phase-separation process based on the predominance at low temperature and low molecularmobility of QP–QP and DMPC–DMPC aggregation forces, prompting the formation of QP-rich domains embedded in a DMPC-rich matrix.High temperature prompts the QP/DMPC ideal mixing.",
author = "Francesco Castelli and Pignataro, {Bruno Giuseppe} and Laura Sardone and Sarpietro, {Maria G.} and Bruno Pignataro and Giovanni Marletta and Francesco Castelli and Giovanni Nicolosi",
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T1 - Temperature and pressure dependence of quercetin-3-O-palmitate interaction with a model phospholipid membrane: film balance and scanning probe microscopy study

AU - Castelli, Francesco

AU - Pignataro, Bruno Giuseppe

AU - Sardone, Laura

AU - Sarpietro, Maria G.

AU - Pignataro, Bruno

AU - Marletta, Giovanni

AU - Castelli, Francesco

AU - Nicolosi, Giovanni

PY - 2004

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N2 - The molecular interaction of quercetin-3-O-palmitate (QP) with dimyristoylphosphatidylcholine (DMPC) has been studied. Film balancemeasurements of the average molecular area vs QP molar fraction in DMPC/QP mixed monolayers showed that relevant positive deviationsfrom ideality, i.e., a less dense monolayer packing, occurred for a temperature of 10◦C, below the critical melting transition temperature ofDMPC monolayers (Tcm≈20◦C), while ideal behavior was observed at 37◦C, above this phase transition temperature. The positive deviationobserved at low temperatures in the average molecular area increased with the surface pressure. Scanning probe microscopy measurementsperformed on mixed monolayers transferred on mica showed that the deviations from ideality were connected to the formation of nanometric-scale QP-rich domains. However, the formation of aggregates was observed only for relatively high-QP molar fractionsXQP0.25 at 10◦C,while it was not observed at 37◦C, i.e., when the ideal mixing was found at the air/water interface. The observed effects are explained in termsof a temperature- and surface pressure-dependent phase-separation process based on the predominance at low temperature and low molecularmobility of QP–QP and DMPC–DMPC aggregation forces, prompting the formation of QP-rich domains embedded in a DMPC-rich matrix.High temperature prompts the QP/DMPC ideal mixing.

AB - The molecular interaction of quercetin-3-O-palmitate (QP) with dimyristoylphosphatidylcholine (DMPC) has been studied. Film balancemeasurements of the average molecular area vs QP molar fraction in DMPC/QP mixed monolayers showed that relevant positive deviationsfrom ideality, i.e., a less dense monolayer packing, occurred for a temperature of 10◦C, below the critical melting transition temperature ofDMPC monolayers (Tcm≈20◦C), while ideal behavior was observed at 37◦C, above this phase transition temperature. The positive deviationobserved at low temperatures in the average molecular area increased with the surface pressure. Scanning probe microscopy measurementsperformed on mixed monolayers transferred on mica showed that the deviations from ideality were connected to the formation of nanometric-scale QP-rich domains. However, the formation of aggregates was observed only for relatively high-QP molar fractionsXQP0.25 at 10◦C,while it was not observed at 37◦C, i.e., when the ideal mixing was found at the air/water interface. The observed effects are explained in termsof a temperature- and surface pressure-dependent phase-separation process based on the predominance at low temperature and low molecularmobility of QP–QP and DMPC–DMPC aggregation forces, prompting the formation of QP-rich domains embedded in a DMPC-rich matrix.High temperature prompts the QP/DMPC ideal mixing.

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